电针“丰隆”穴对高脂血症大鼠巨噬源性泡沫细胞形成及其胆固醇外流的影响

Effects of electroacupuncture at “Fenglong” (ST 40)on formation of macrophage-derived foam cell and efflux of cholesterol in hyperlipidemia rats

目的:探讨电针"丰隆"穴治疗高脂血症的作用机制。方法:将40只SPF级SD大鼠随机分为正常组(A组)、模型组(B组)、模型饮控组(C组)、模型加电针组(D组)及模型饮控加电针组(E组),每组8只。A组大鼠连续普通饲料喂养,其余4组用高脂饲料饲喂法建立高脂血症模型后,C组、E组改用普通饲料喂养,同时D组、E组电针"丰隆"穴治疗,每次30min,每日1次,治疗28d后,提取各组大鼠腹腔巨噬细胞,用油红O染色检测泡沫细胞形成及酶联免疫吸附法(ELISA)检测巨噬细胞内总胆固醇(TC)含量,分析其胆固醇外流率。结果:B组、C组、D组大鼠油红O染色阳性细胞计数、巨噬细胞内TC含量较A组均显著增加(均P<0.01),C组、D组、E组阳性细胞计数、细胞内TC含量较B组显著减少(均P<0.01),其中D组较C组下降明显(均P<0.01)。与C组、D组比较,E组阳性细胞计数、TC含量明显减少(均P<0.01),与A组之间差异无统计学意义(P>0.05)。同时,与C组比较,D组、E组巨噬细胞内胆固醇外流率显著增加(均P<0.01),且E组较D组增加明显。结论:电针"丰隆"穴能显著抑制巨噬细胞向泡沫细胞转化,提高巨噬细胞内胆固醇外流率,可预防和逆转泡沫细胞形成,对于治疗高脂血症和防止其进一步发展有一定的作用。

Objective To explore action mechanism of electroacupuncture （EA） at ＂Fenglong＂ （ST 40） for treatment of hyperlipidemia. Methods Forty SPF-grade SD rats were randomly divided into a normal group （group A）, a model group （group B）, a model diet-control group （group C）, a model ＋ EA group （group D） and model diet-control ＋ EA group （group E）, 8 cases in each one. The rats in group A were fed with normal diet continuously while those in the rest 4 groups were fed with high-fat diet to establish hyperlipidemia model. After- wards, the rats in group C and group E were fed with normal diet, while EA at ＂Fenglong＂ （ST 40） was applied in group D and group E, 30 min per time, once a day. After 28 days of treatment, macrophage was collected in each group. Oil red O-staining was applied to detect the formation of foam cells, and enzyme-linked immunosor- bent assay （ELISE） was adopted to measure the contents of total cholesterol （TC） in macrophage and analyze the rate of cholesterol efflux. Results The counts of positive cells of oil red O-staining and the contents of TC in the group B, group C and group D were significantly increased compared with those in the group A （all P〈O. 01）. The counts of positive cells and contents of TC in the group C, group D and group E were significantly clecreased compared with those in the group B （all P〈0.01）, and the decline in group D was more obvious than that in the group C （all P〈O. 01）. Compared with group C and group D, the counts of positive cells and contents of TC in the group E was obviously decreased （all P〈0. 01）, which was not statistically different from group A （P〈 0.05）. In the meantime, compared with group C, the rate of cholesterol efflux in group D and group E was signifi- cantly increased （both P〈0.01）, and the rise in group E was more obvious than that in the group D. Conclusion The electroaeupuncture at ＂Fenglong＂ （ST 40） could significantly prohibit the transformation of macrophage intofoam cell and increase rate of cholesterol efflux in macrophage, which could prevent and reverse the formation of foam cell and play an essential role in treating hyperlipidemia and stopping it from developing into a further level.