胸腺肽α1对小鼠机体免疫系统的影响

Effects of Thymosin α1 on Immune Effector Molecules of Mouse

目的探讨胸腺肽α1(Tα1)对正常小鼠机体相关免疫效应分子的作用及胸腺肽α1诱导特异性免疫耐受的机制。方法将BABL/c小鼠分为4组,正常对照组(无任何药物,n=5)、环孢素A(CsA)组(给予10mg/kg CsA,n=20)、Tα1组(给予400μg/kg Tα1,n=20)、CsA+Tα1组(CsA、Tα1剂量同前,n=20),每日腹腔注射1次,共14d。分别于用药后第1、7、14、21d各取5只(正常对照组取1只)采血取血清行液相芯片细胞因子(反映Th1、Th2和Th17功能)检测。处死小鼠后取脾脏,制备小鼠脾细胞悬液进行淋巴细胞培养,仍分为4组,除对照组外,其余3组分别加入0.25mg/mL CsA、10μg/mL Tα1、0.25mg/mL CsA+10μg/mL Tα1,培养3d后检测细胞的生长增殖,以及培养上清液细胞因子的表达。结果 BABL/c小鼠给予Tα1、CsA后,与正常对照组比较,白介素(IL)-1a、IL-2、IL-6、IL-17等炎性细胞因子降低(P<0.05),而抑炎因子IL-10升高(P<0.05),CsA和Tα1对细胞因子表达的影响相似(不同时间点及不同细胞因子两单药组间差异均无统计学意义),CsA和Tα1联合用药可加强单用药作用。淋巴细胞培养3d后,Tα1、CsA、CsA+Tα1组淋巴细胞增殖受抑制,与正常对照组比较,差异有统计学意义;培养细胞上清液细胞因子表达与正常对照组比较,各组IL-1α、IL-6表达下降,IL-10升高,差异均有统计学意义(P<0.05),而IL-2、IL-17各组间差异无统计学意义。结论 Tα1对小鼠正常机体免疫功能具有一定的调节作用,促使T细胞由Th1向Th2转化。

Objective To analysis the effects of Tal on the immune effector molecules in mouse immune system. Methods Sixty five BABL/c mice were divided into four groups： CsA group （n=20）, Tα1 group （n=20）, CsA＋Tα1 group （n=20） and control group （n=5）, In the 3 experimental groups, 10 mg/kg CsA, 400 μg/ kg Tα1, 10 mg/kg CsA＋400 μg/kg Tal were respectively administrated by intraperitoneal injection daily. Luminex was performed for cytokine detection at 1 d, 7 d, 14 d, 21 d day after the above treatments. Lymphocyte culture was prepared with the mouse spleen suspension, and then treated with 0. 25 mg/mL CsA, 10μg/mL Tα1 or 0.25 mg/mL CsA＋10μg/mL Tα1 in vitro, respectively. Three days later, OD values of each treated lymphocyte culture and several cytokines in the culture were measured. Results Compared with other groups, CsA＋Tal group had significant lower IL-1α, IL-2, IL-6, IL-17, and significant higher IL-10 at 1 d, 7 d, 14 d, 21 d after the treatments （P〈0.05）. Three days after the culture, OD value in the control group was significantly higher than that in Tα1 group, CsA group, and CsA＋Tα1 group （P〈0. 05）. IL-1α and IL-6 in the control group were significantly higher than those in the experiment groups （P 〈 0. 05）, while IL-10 in the control group was significantly lower than that in the experiment groups （P〈0.05）. IL-2 and IL-17 were similar. Conclusion Tα1 show regulatory effect on the immune effector molecules which could promote Thl cells transforming to Th2 cells.