摘要
目的观察18kDa转位蛋白(TSPO)在异氟醚麻醉所致的大鼠认知功能损伤中的神经保护作用。方法选取4月龄SD雄性青年大鼠10只,随机分为青年对照组(AC组)和青年麻醉组(AA组);20月龄SD雄性老年大鼠10只,随机分为老年对照组(OC组)和老年麻醉组(OA组)。麻醉组吸入2%异氟醚6h,对照组吸入空气6h。麻醉后1d,2d,3d,4d进行水迷宫测试,检测海马组织TSPO蛋白表达。测定麻醉前(T1)、麻醉诱导6h(T2)、麻醉后24h(T3)、麻醉后48h(T4)血清TNF-α,IL-1β含量。结果与对照组比较,吸入异氟醚后大鼠逃避潜伏期延长(P<0.05),老年大鼠延长明显(P<0.01);麻醉后大鼠海马区TSPO蛋白表达增加(P<0.05),老年大鼠增加显著(P<0.01);与T1相比,T2时大鼠血清TNF-α,IL-1β水平均升高(P<0.05),T3,T4时逐渐降至正常水平。结论异氟醚可能通过诱发神经炎症导致大鼠认知功能下降,而其认知功能的恢复可能与海马区TSPO蛋白表达增加有关。
Objective To investigate the neuroprotection effect of （18kDa） translocator protein（TSPO） on isoflurane inhalation induced-cognitive deficit in rats.Methods Ten adult male Sprague-Dawley（SD）rats（4 months） were randomly allocated into two groups （n=5 each）：adult control group（AC）,adult anesthesia group（AA）.Another ten old male SD rats（20 months） were randomly allocated into two groups（n=5 each）：old control group（OC）,old anesthesia group（OA）.Each anesthesia group were anesthetized with 2%isoflurane for 6h.Each control group breath air for 6h.All the rats were trained for 3 days before anesthesia ,and tested in morris water maze for 4 days after anesthesia .The rats were killed immediately after the tests and hippocampus was isolated for determination of protein TSPO expression using western-blot technique.Blood was taken from caudal vein before anesthesia （T1）,and 6h（T2）,24h（T3）,48h（T4） after anesthesia,for de-termination of the content of serum TNF-α,IL-1βusing ELISA.Results Compared with control group,escape latency became longer after Isoflurane anesthesia（P〈0.05）,especially in old group（P〉0.01）.TSPO expression in hippocampus increased （P〈0.05）,signicantly in old group（P〈0.01）.The content of serum TNF-α,IL-1βat T2 was more than that at T1（P〈0.05）,and then decreased to normal gradually at T3,T4.Conclusion Cognitive function declined after neuroinflammation induced by Isoflurane ,and the recovery of which may be related to the increasing expression of TSPO in rat hippocampus .
出处
《潍坊医学院学报》
2014年第2期129-131,I0002,共4页
Acta Academiae Medicinae Weifang
基金
山东省教育厅资助课题(课题编号:J13LL02)
