兔胃肠黏膜摄氧能力的研究

Research on Rabbit Gastrointestinal Mucous Membrane Oxygen Uptake Capacity

目的：观察兔胃肠黏膜摄氧能力及充氧量对其摄氧能力的影响。方法新西兰白兔80只，采用随机数字表法，将白兔随机分为4组（n＝20）：对照组（C组），实验组1（E1组），实验组2（E2组）和实验组3（E3组）。所有白兔均插胃管，麻醉后开腹游离出门静脉，并于此时（ T0）分别抽取门静脉血和股静脉血进行血气分析。 C组注入氮气50ml，E1组经胃管注入氧气20ml，E2组经胃管注入氧气50ml，E3组经胃管注入氧气80ml。4组均于充气后5min（T1），10min（T2），30min（T3），60min（T4）分别抽取门静脉血和股静脉血进行血气分析。结果 C组注氮气前后门静脉氧分压变化无统计学意义，股静脉氧分压变化无统计学意义（ P＞0．05）；与C组比较，E1组充氧气前后门静脉和股静脉氧分压变化均无统计学意义；与C组比较，E2组充氧气前后门静脉氧分压T1，T2时升高（P＜0．05），T3，T4时无统计学意义（P＞0．05），股静脉氧分压T1～T4时变化无统计学意义（P＞0．05）；与E2组比较，E3组门静脉和股静脉氧分压T1～T4时无统计学意义（ P＞0．05）。结论兔胃肠道黏膜具有摄氧能力且与氧气压力呈正相关，兔胃黏膜在充氧容积超过80ml后摄氧能力出现“封顶效应”。

Objective To observe the rabbit gastrointestinal mucous membrane oxygen uptake ability and oxygen filling its influ -ence on the oxygen uptake capacity .Methods Eighty New Zealand white rabbits ,using the method of random number table ,were randomly divided into four groups（n=20）：the control group（group C）,the experimental group 1（E1）,group 2（E2）,and the experimental group 3 group（E3）.All the white rabbit accepted a gastric tube ,and portal veins were dissociate after laparotomy ,and at this time（T0）,portal venous blood and femoral venous blood were extracted for blood gas analysis respectively .Group C accepted nitrogen 50ml by stomach tube into ,E1 group oxygen 20 ml,E2 group oxygen 50ml,E3 group 80ml.Four groups all within 5min after the inflatable（T1）,10min（T2）,30min（T3）, 60min（T4） extraction of portal venous blood and femoral venous blood of all subjects were extracted for blood gas analysis in 5min（T1）, 10min（T2）,30min（T3）,60min（T4） after inflation,respectively.Results Portal venous oxygen partial pressure changes before and after the nitrogen injection has no statistical significance in group C ,as well as the femoral vein oxygen partial pressure （ P〈0.05）.Compared with group C,portal vein and femoral venous oxygen partial pressure changes before and after oxygen filling had no statistical significance in E 1 group（P〈0.05）.Compared with group C,in E2 group,before and after portal vein oxygen partial pressure of oxygen filling was elevated in T1,T2（P〈0.05）,but T3,T4 had no statistical significance（P〈0.05）,but femoral venous oxygen partial pressure in T1~T4 change noted no statistical significance（P〈0.05）.Compared with E2 group,E3 group of portal vein and femoral vein oxygen partial pressure T 1~T4 had no statistical significance（P〈0.05）.Conclusion Rabbit gastrointestinal mucosa with oxygen uptake ability and positively are correlated with oxygen pressure ,and rabbit gastric mucosa after oxygen filling volume of more than 80ml,oxygen uptake ability appear “ceiling effect”.