白介素7在原发性干燥综合征患者唇腺组织中的表达及其临床意义

Expression and Clinical Significance of Interleukin-7 in the Labial Gland Tissue of Patients with Primary Sjgren's Syndrome

目的探讨白介素7(IL-7)在原发性干燥综合征(pSS)患者唇腺组织中的表达及其与唇腺组织中淋巴细胞浸润和红细胞沉降率(ESR)、C反应蛋白(CRP)、免疫球蛋白的相关性。方法选择2005年3月—2012年10月滨州市人民医院风湿免疫科门诊及住院的pSS患者30例为pSS组,另选择同时期颌面部创伤和唇腺囊肿患者5例为对照组。采用免疫组织化学法检测唇腺组织中IL-7的表达情况,采用HE染色法检测唇腺组织中淋巴细胞浸润情况。分析IL-7与唇腺组织中淋巴细胞浸润及ESR、CRP、免疫球蛋白的相关性。结果 IL-7阳性产物定位于胞质,呈棕黄色,主要表达于导管、腺泡上皮细胞及淋巴细胞浸润灶。pSS组29例呈阳性表达,阳性表达率为97%,对照组1例呈阳性表达,两组IL-7阳性表达率比较,差异有统计学意义(P=0.013)。IL-7阳性细胞数与淋巴细胞浸润灶数呈正相关(r=0.807,P<0.01),与ESR呈正相关(r=0.546,P<0.01),与CRP、IgG、IgM、IgA无相关性(P>0.05)。结论 IL-7在pSS患者唇腺组织中呈高表达,表达情况与淋巴细胞浸润及ESR相关,高表达的IL-7可能在pSS唇腺组织的破坏及发病过程中起重要作用。

Objective To study the expression of interleukin - 7 （ IL - 7 ） in the labial gland of patients with primary Sjsgren＇s syndrome （pSS） and its relationship with the infiltration of lymphocytes in the gland, serum erythrocyte sedimentation rate （ ESR）, C - reactive protein （CRP） and immunoglobulin. Methods 30 pSS inpatients and outpatients from the Department of Rheumatism of Binzhou People＇s Hospital from March 2005 to October 2012 were selected as pSS group, while 5 patients with maxillofacial trauma and labial gland cyst of the same period were selected as control group. Immunohistocbemical method was used to detect the expression of IL - 7 in labial gland, and HE staining was used to detect the lymphocyte infiltration in labial gland. The relationship between IL - 7 and infiltration of lymphocytes in the gland, serum ESR, CRP and immunoglobulin was analyzed. Results IL -7 was expressed in the acini, ducts and lymphocytes infiltration area in pSS. 29 cases of pSS group had positive expression, and the positive expression rate of IL -7 was 97%. One case of the control group had positive expression, and the positive expression of IL -7 between the two groups showed statistically significant difference （P = 0. 013 ） . The positive cells of IL -7 positively were correlated with the lymphocyte infiltration focus and ESR （ r = 0. 807, 0. 546, P 〈 0.01 ）, but were not correlated with CRP, IgG, IgM and IgA （P 〉 0. 05） . Conclusion The elevation of IL - 7 expression in labial gland in patients with pSS is related to the infiltration of lymphocytes and ESR, which plays an important role in the process of glandular destruction and in the pathogenesis of pSS.