湖南岳阳地区柑橘黄龙病PCR检测

PCR Detection of Citrus Huanglongbing Pathogen in Yueyang of Hunan Province

为确诊近年在湖南岳阳部分柑橘产区发生的一种使柑橘叶片斑驳、果实变小及全株矮化,甚至枯死的传染性病害,摸清病源来源,采用多聚酶链式反应(PCR)技术对该地区出现的柑橘黄龙病疑似症状植株进行了检测。结果表明,岳阳华容县南山乡、华容胜峰乡、华容西湖乡从福建黄龙病疫区引进的琯溪蜜柚,PCR扩增带谱为563 bp,与阳性对照一致,存在柑橘黄龙病病菌;在岳阳麻糖春风同一果园中以宫本为基砧高接了琯溪蜜柚(福建引进)的植株,PCR扩增带谱为563 bp,与阳性对照一致。未高接换种宫本无扩增带谱,与阴性对照一致,无柑橘黄龙病病菌;由岳阳本地繁殖的苗木椪柑、脐橙、未高接宫本无扩增带谱,与阴性对照一致,无黄龙病危害。通过本研究可知,湖南岳阳地区存在柑橘黄龙病危害,并为首次发现,但病源主要来源福建黄龙病疫区调进的未脱毒琯溪蜜柚植株,目前传播方式主要是高接放种。

In recent years, diseased plants of citrus have been found in Yueyang County, Hunan Province. The symptoms of the diseased trees include leaf mottle, small fruit and stunting, serious whole wither. The symptom is similar to citrus Huanglongbing. In order to make a definite diagnosis of this disease, take the control measure in time, this research employed PCR technology detecting the disease. The PCR test results suggested that, Guanximiyou pomelos of Yueyang Huarong Nanshan County and Huarong Shenfeng County and Huarong Xihu County were infected by citrus Huanglongbing Pathogen. The Guanximiyou pomelos came from Fujian Province. In Chunfeng Garden Matang County, citrus topworked by Guangximiyou polemo was infected by citrus Huanglongbing Pathogen, but untopworked Citrus reticulata Blanco cv. miyamoto was uninfected. Citrus from Yueyang was not infected by citrus Huanglongbing Pathogen. Citrus Huanglongbing pathogen was by detected from the citrus in Yueyang, that was the first discovered. Citrus Huanglongbing pathogen was from Guanximiyou pomelo of Fujian Province. Citrus Huanglongbing pathogen infect citrus by topworking and importing disease. A little citrus were infected by citrus Huanglongbing in Yueyang. Diseased citrus were Guanximiyou polemo from Fujian Province and Citrus reticulata Blanco cv. miyamoto base stock, other citrus were not infected by citrus Huanglongbing pathogen in Yueyang.