甘蔗ShSAP1基因的启动子克隆与序列分析

Cloning and Sequence Analysis of ShSAP1 Promoter from Sugarcane

启动子在转基因育种中具有重要地位,为了发掘高效的胁迫诱导和组织特异型启动子,提高目的基因的表达效率,根据甘蔗逆境相关蛋白家族基因ShSAP1的基因组序列,利用Genome Walking法对ShSAP1的启动子进行了分离,并对其序列进行了生物信息学分析。分离获得了1243 bp的启动子序列,该序列具有启动子的核心元件,还包含了干旱等胁迫相关的应答元件、MYB/MYC转录因子识别与结合元件、光应答元件及组织特异性相关元件,说明ShSAP1的启动子可能具有逆境应答及组织特异表达特性,值得开展进一步的功能研究。

Promoter play an important role in transgenic breeding, discovering of active promoters, especially stress-specific and organ/tissue-specific promoter is of great interest to manage the concerns associated with gene expression efficiency. According to the genome sequence of ShSAP1, a member of stress associated proteins （SAPs）, a 1243 bp promoter region of ShSAP1 was isolated from sugarcane genome by genome walking. Sequence analysis found the promoter region contained core promoter elements and other ciselements like drought and other stress response elements, MYB/MYC recognition and binding site, light responsive elements and tissue specific expression elements, which indicated SbSAP1 promoter might be a stress-specific and tissue-specific promoter. The function of SbSAP1 promoter still needs further study.