贻贝粘蛋白创面修复敷料蛋白质含量分析方法的研究

Determination of Protein Content in Mussel Adhesive Protein Wound Repair Dressings

目的建立贻贝粘蛋白创面修复敷料中蛋白质含量的测定方法。方法采用Lowry法测定创面修复敷料中蛋白质的含量。结果蛋白质浓度在9.5-190.0 μg·mL^-1范围内呈线性关系,回归方程为Y=1.39×10^-2 X＋3.9×10^-3,r=0.999。加样回收率为102.8%-103.2%,RSD为0.13%-0.37%。结论 Lowry法测定创面修复敷料中蛋白质含量经方法学验证,可用于创面修复敷料类产品中蛋白质含量的检测和质量控制。

Objective To establish a quantitative analytic method for determination of protein content in mussel adhesive protein （MAP） wound repair dressings. Methods Lowry＇s method was used for determination of protein contents in MAP wound repair dressings. Results The calibration curves of protein were linear in the range of 9.5-190.0 μg ·mL-1 （r= 0. 999）. The average recoveries at three spiked levels were 102.8%-103.2% with relative standard deviations of 0.13%-0. 37%. Conclusion Methodology validation proves that the method can be used for determination of protein in MAP wound repair dressings.