髓样分化因子88在子痫前期患者血浆中的表达

Expression of myeloid differentiation factor 88 in plasma of patients with eclampsism

目的探讨髓样分化因子88(MyD88)在子痫前期患者血浆中的表达及其与子痫前期的关系。方法 32例子痫前期患者分为轻度子痫前期组和重度子痫前期组,每组16例,选择同期正常晚期孕妇16例作为对照组。采用反转录聚合酶链反应和酶联免疫吸附测定法检测血浆中MyD88 mRNA和蛋白水平。结果轻度和重度子痫前期组患者血浆中MyD88 mRNA及蛋白水平显著高于对照组,差异均有统计学意义(P<0.05);轻度子痫前期组与重度子痫前期组患者血浆中MyD88 mRNA及蛋白水平比较差异无统计学意义(P>0.05)。结论 MyD88可能参与了子痫前期的发病过程。

Objective To explore the relationship between the expression of plasmic myeloid differentiation factor 88 （ MyD88 ） and eclampsism. Methods Thirty-two patients with eclampsism were divided into mild eelampsism group and se- vere eclampsism group, sixteen cases in each group. Sixteen normal late pregnant women were selected as control group. The levels of MyD88 mRNA and protein in plasma were detected by reverse transcription polymerase chain reaction and enzyme- linked immunosorbent assay. Results The levels of plasmic MyD88 mRNA and protein in mild and severe eclampsism group were significantly higher than those in control group （ P 〈 0.05 ）. There was no significant difference in the level of plasmic MyD88 mRNA and protein between mild eciampsism group and severe eclampsism group （ P 〉 0.05 ）. Conclusion Myd88 may play an important role in the occurrence and development of eclampsism.