采用IV型胶原酶建立小鼠脑出血模型

An animal model of collagenase type IV-induced intracerebral hemorrhage in mice

目的:用脑内注射胶原酶方法建立小鼠脑出血模型。方法:在动物脑立体定位仪下将不同剂量Ⅳ型胶原酶0.5μl注射到昆明小鼠左侧纹状体处,分别在术后24h对小鼠运动功能进行检测,并对出血部位脑组织进行尼氏染色,光镜观察染色结果来验证模型制作是否成功。结果:术后小鼠即表现右侧肢体运动功能障碍。形态学观察证实左侧纹状体内血肿形成,且在一定范围内,出血灶大小与肢体偏瘫症状发展过程基本一致。不同个体间相同单位的胶原酶出现的血肿体积和部位基本一致。结论:应用Ⅳ型胶原酶脑内注射方法建立的小鼠脑出血模型,是一种方法简单,效果可靠,易于大批量制作的动物模型。脑内注射0.02U的IV型胶原酶制备的脑出血模型能较好满足实验要求。

Objective：To establish a typical mouse model of intracerebral hemorrhage （ICH） .Methods ：Intracerebral hemorrhage in mice were generated by stereotaxically injecting different dose of collagenase type Ⅳ into the left corpus striatum .Every mouse received a volume of 0 .05μl collagenase type Ⅳ .Neurological scores were as-sessed and recorded 24 hours after injection .After behavioral testing the brains were processed for routine histologi-cal examination .Light microscope observation to determine if the animal model was successful .Results：Mice showed the motor dysfunction after operation .Morphology confirmed that the hematoma formed at the left corpus striatum ,and within a certain range ,the size of hemorrhage and the development of symptoms of hemiplegia was re-lated .Different individuals of the same unit of collagenase type Ⅳmaking the volume and location of hematoma was same .Conclusion ：Intracerebral hemorrhage can be induced in mouse by injecting collagenase type Ⅳ into the corpus striatum of the brain .This is a useful economical and reproducible method to make intracerebral hemorrhage .ICH model was made by intracerebral injection of 0 .02U type IV collagenase can meet our experimental requirements .