摘要
以高效液相色谱法同时测定四方藤中岩白菜素和白藜芦醇的质量分数,色谱柱为SinoChrom ODSBP C18(5 μm,4.6 mm×200 mm),流动相为乙腈-水,流速为1.0 mL· min-1,在波长为318 nm下进行梯度洗脱.结果表明,岩白菜素和白藜芦醇对照品分别在0.380 95~1.944 76 μg(R2=0.999)、0.019 867~0.099 33 μg(R2 =0.999 5)呈良好的线性关系,平均加样回收率分别为97.71%、97.53%;RSD分别为1.08%(n=6)、0.88%(n=6).该方法操作简单、重现性好、结果准确,能在318 nm处对四方藤中岩白菜素和白藜芦醇的质量分数同时进行定量分析.
To develop a quantitative method for determining bergenin and resveratrol simultaneously in Cissus pteroclada Hayata by HPLC, the determination is performed in gradient separations with a column SinoChrom ODS-BP C18(5μm, 4. 6 mm× 200 mm) and solvent system consisting of water- acetonitrile. The flow rate is 1.0 mL· min-1 and the wavelength at 318 nm. The standard of curves of bergenin and resveratrol showed a good linearity. For bergenin(R2 =0. 999 0) in the range of 0. 380 954 1. 904 76μg,the average recovery was 97.71% (RSD= 1.08%). For resveratrol(R2 =0. 999 5) in the range of 0.019 867~0.099 33μg,the average recovery was 97.53%(RSD=0.88%). The method is simple, reproducible and accurate,which can be simultaneously used for quantitative analysis of bergenin and resveratrol in Cissus Pterolada Hayata at 318 nm.
出处
《广西师范大学学报(自然科学版)》
CAS
北大核心
2014年第1期91-94,共4页
Journal of Guangxi Normal University:Natural Science Edition
基金
国家自然科学基金资助项目(21262006)
广西医药人才小高地资助项目(1107)
药用资源化学与药物分子工程教育部重点实验室开放基金资助项目(桂科能07109001-14)