气相色谱-飞行时间质谱法分析德国洋甘菊油中的挥发性成分

Analysis of Volatile Components in German Chamomile Oil by GC-TOF MS

利用气相色谱-飞行时间质谱,对德国洋甘菊油的挥发性成分进行了分析,并用峰面积归一化法计算了各成分的相对含量。通过质谱库检索,辅助英文版精油数据库(ESO2010版)比对,分析并确定出洋甘菊油中的72个成分,占其总挥发性成分的88.35%。德国洋甘菊油的主要成分为α-红没药醇氧化物B(16.17%)、双环大根香叶烯(15.35%)、α-红没药醇氧化物A(10.11%)、蒿酮(8.23%)、大根香叶烯D(7.01%)、α-红没药酮氧化物(6.08%)、β-罗勒烯(5.39%)、(E)-β-金合欢烯(2.56%)、斯巴醇(2.48%)、母菊薁(2.46%)、香芹酮(1.49%)、薄荷醇(1.37%)等。其中萜类化合物及其衍生物共42个,占洋甘菊油总挥发性成分的79.99%。通过保留指数来鉴别同系物及同分异构体,提高了天然香原料中化合物定性的准确性。

The volatile components of chamomile oil were analyzed by gas chromatography/time-of-flight mass spectrometry,and the peak area normalization method was used to calculate the relative content of each component.With the mass spectrometry library search,72 compounds which account for 88.35％ of the volatile components of chamomile oil,were identified by using reference literature and retention index of ESO database （ESO 2010）.α-Bisabolol oxide B （16.17 ％）,bicyclogermacrene （15.35％）,α-bisabolol oxide A （10.11％）,artemisia ketone （8.23％）,germacrene D （7.01％）,α-bisabolone oxide （6.08％）,β-ocimene （5.39％）,（E）-β-farnesene （2.56％）,spathulenol （2.48％）,chamazulene （2.46％）,carvone （1.49％）and menthol （1.37％） were the main components.Among them,42 compounds were terpenoids and their derivatives,which account for 79.99 ％ of the volatile components of chamomile oil.The homologues and isomers were confirmed by using retention index,and the accuracy of compound qualitative analysis in natural flavor was improved.