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一种FRET多肽的酶-化学法合成

Enzyme-chemical Synthesis of a FRET Peptide
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摘要 选择FITC和罗丹明类荧光分子Alexa Fluor（R）514作为荧光对，通过酶-化学方法合成了一种潜在的FRET多肽．首先，在OGT催化下，将N-叠氮乙酰葡糖胺（GlcNAz）通过O-糖苷键连接到FITC标记的底物八肽（YAVVPVSK）的丝氨酸羟基上；然后，将炔基标记的Alexa Fluor（R）514通过点击化学反应连接到GlcNAz上，得到目标化合物，并通过质谱进行了表征． A chimerical peptide with a fluorescence resonance energy transfer （FRET） pair, Alexa Fluor（R） 514 and FITC-tagged, was synthesized via enzyme-chemical method. First- ly, an octapeptide （YAVVPVSK） was labeled with fluorescein （FITC） at C- or N-terminus, which was linked with GlcNAz via sOGT mediated glycosyl-transferring reaction. Then Fluo- rescein Alexa Fluor（R） 514 was linked to the azide group via click reaction. The resultant molecule was identified via MALDI-TOF-MS.
作者 刘晓燕 王玉秋 何晔 周丰 郭祖浩 张连文 李玲
机构地区 南开大学药学院
出处 《南开大学学报（自然科学版）》 CAS CSCD 北大核心 2014年第2期98-103,共6页 Acta Scientiarum Naturalium Universitatis Nankaiensis
基金 国家自然科学基金(30970644)
关键词 O-GLCNAC OGT 点击化学 荧光能量共振转移 O-GIeNAc OGT click chemistry reaction FRET
分类号 O657 [理学—分析化学]
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参考文献22

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南开大学学报（自然科学版）
2014年 第2期

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