摘要
目的：建立反相高效液相色谱（RP-HPLC）法测定维生素C咀嚼片的含量.方法：采用Agilent ZORBAX SB-C18色谱柱（4.6 mm×250 mm,5 μm）,流动相为0.02 mol/L磷酸二氢钾溶液（用磷酸调节pH至3.0）-甲醇（95∶5）;流速为0.6 ml/min;波长为244 nm;柱温为30℃.结果：维生素C在2.098 ～ 62.94 μg/ml浓度范围内线性关系良好（Y=52 182X-6 092.4,r=1.0000）,辅料无干扰,回收率为97.5％,RSD为1.2％（n=9）.结论：本方法操作简便、快捷,可为该制剂提供严格可控的质量标准.
OBJECTIVE：To establish RP-HPLC method for determination of Vitamin C chewable tablet.METHODS：Agilent ZORBAX SB-C18 chromatographic column was applied with mobile phase consisting of 0.02 mol/L potassium dihydrogen phosphate （adjusted pH to 3.0 with phosphoric acid）-methanol（95 ∶ 5） at flow rate of 0.6 ml/min,with wavelength of 244 nm and column temperature of 30 ℃.RESULTS：The linear range of vitamin C was 2.098-62.94 μg/ml （Y =52 182X-6 092.4,r =1.000 0） which was free of interference of the excipients.The average recovery was 97.5％ with RSD of 1.2％ （n =9）.CONCLUSIONS：The method is simple and rapid and it provides controllable quality standard for Vitamin C chewable tablet.
出处
《中国医院用药评价与分析》
2014年第4期323-325,共3页
Evaluation and Analysis of Drug-use in Hospitals of China
