HMGBl和β-catenin的表达在非小细胞肺癌中的临床病理研究

Clinical pathological study of HMGBl and β-catenin expression in non-small cell lung cancer

目的:高迁移率族蛋白B1(HMGBl)和β-连环蛋白(β-catenin)的表达在非小细胞肺癌中的临床病理的关系。方法:将48例非小细胞肺癌(NSCLC)患者的病理标本分为鳞癌组20例,腺癌组20例及大细胞癌组8例,选取正常肺组织切片20例作为正常组,用免疫组化法染色,试剂选用抗HMGB兔抗人多克隆抗体及β-catenin鼠抗人单克隆抗体,以1∶100的比例稀释。用SP法测定HMGBl、HMGB1mRNA的表达水平及β-catenin的胞质表达水平,用Image-Pro Plus图像分析系统测量HMGBl的光密度值,用确定的NSCLC切片为阳性对照.磷酸缓冲液代替一抗作阴性对照。结果:3组HMGBl光密度值和HMGB1mRNA的转录水平明显高于正常组,差异具有统计学意义(P<0.05);而3组HMGBl光密度值的组间比较,差异无统计学意义(P>0.05);3组HMGB1mRNA的转录水平的组间比较,鳞癌组高于腺癌组及大细胞癌组,差异具有统计学意义(P<0.05);正常组与3组β-catenin的胞质表达的比较,差异具有统计学意义(P<0.05)。结论:HMGBl、β-catenin与NSCLC的发病有相关性,HMGBl及β-catenin均参与了NSCLC的发病,HMGB1mRNA在鳞癌的转录水平高于腺癌及大细胞癌,而β-catenin与NSCLC的病理学分类无关。

Objective： To explore the clinical pathological relationship of HMGB1 and β-catenin expression in non-small cell lung cancer. Methods： Pathologic specimens of 48 non-small cell lung cancer （NSCLC） were collected and divided into squamous cell carcinoma group （20 cases）, adenocarcinoma group （20 cases）, and large cell carcinoma （8 cases）. Twenty specimens of normal lung tissue were also collected. Resistance HMGB rabbit polyclonal antibody and β-catenin rat monoclonal antibody were chose to dilute at the proportion of 1 100. SP method was used to determine the expression level of HMGBlmRNA and β-catenin cytoplasmic expression level. HMGB1 optical density value was measured with Image - Pro Plus Image analysis system. Certain NSCLC slice was set as positive control. Phosphate buffer instead of primary antibodies was set as a negative control. Results. The HMGB1 optical density value and HMGBlmRNA transcription level of the three groups of NSCLC are significantly higher than normal group; but the HMGB1 optical density value was not significant different among the three groups （P〉0.05）. As for the HMGBlmRNA transcription level, it was higher in the squamous carcinoma group than that in the large cell carcinoma group （P〈0.05）. β-catenin cytoplasmic expression was significant different between the control group and three NSCLC groups. Conclusion. HMGB1 and β-catenin play sig- nificant role in the occurrence of NSCLC. Both HMGB1 andlβ-catenin are involved in the pathogenesis of NSCLC. The transcription level of HMGBlmRNA in squamous cell carcinomas is higher than that of ade- nocarcinoma and large cell carcinoma, and β-catenin is not correlated with pathological type of NSCLC.