吖啶橙诱导酪氨酸酶构筑酚类碳毡传感器及电化学行为

Construction of acridine orange induced tyrosinase-carbon felt-based phenol biosensor and its electrochemical behaviour research

酚类化合物是很多行业的生产废物,其残留对于人类的健康十分不利。因此,对环境中酚类化合物残留的快速准确地检测十分必要。研究使用酪氨酸酶为生物组分,通过吖啶橙诱导将其吸附在碳毡电极上,以环境污染物邻苯二酚及4-氯苯酚为底物,检测所制备电极的性能。研究表明,吖啶橙能够提高酪氨酸酶碳毡电极的性能,稳定及提高酪氨酸酶在碳毡电极表面的活性,对邻苯二酚及4-氯苯酚具有很好的检测性能。

The phenol compounds are important contaminations generated from industrial waste products, which is causing problems for our living environment and showing adverse effects on human. It is necessary to build a rapid and accuracy detection for the environmental phenol compounds. In this research, the tyrosinase （TYR） adsorbed on the carbon felt （CF） electrode by co-adsorbed with acridine orange （AO）. The AO induced TYR-CF electrode is used to detect catechol and 4-chlorophenol. The results show that the presence of AO can enhance the characteristics of TYR-CF electrode, and increase the activity of TYR on the CF surface. It has a good detection function for catechol and 4-chlorophenol.