大鼠肝细胞微球皮下移植生成肝组织初探

Hepatic tissue generation in rats after subcutaneous transplantation of hepatic spheroids

目的 探讨大鼠肝细胞微球皮下移植生成肝组织的可行性，为构建组织工程肝寻找新的方法。 方法 新鲜分离的SD大鼠肝细胞接种在琼脂糖包被的培养皿上进行培养，待其形成肝细胞微球后采用直接注射方式植入大鼠腹股沟处皮下腔。1 周后取腹股沟处组织，HE 染色法观察生成的肝组织形态。 结果 体外培养3 d，肝细胞形成致密的肝细胞微球，直径大小在43 ～ 185 μm，HE 染色显示微球中的肝细胞保持天然肝细胞形态特征。将其植入大鼠腹股沟处皮下腔1 周，HE 染色显示植入的肝细胞成活，并呈三维肝样组织团，肝细胞保持其特有形态特征：胞体为圆形，核大而圆，多为双核。 结论 肝细胞微球皮下移植可形成肝组织，为组织工程肝的构建创建了新的方法。

Objective To seek for the new method for constructing engineered liver tissue by studying the feasibility of generating hepatic tissue in rats after subcutaneous transplantation of hepatic spheroids. Methods Hepatic cells freshly isolated from SD rats were cultured in an agar-coated culture dish. One week after the generated hepatic spheroids were transplanted into the subcutaneous space of groin in rats by direct injection, groin tissue taken from the rats was stained with H＆E to observe its morphology. Results Dense hepatic spheroids were generated after 3 days of culture with a diameter of 43-185 μ m. H＆E staining showed the natural morphology of hepatocytes in hepatic spheroids. One week after the hepatocytes were transplanted into the subcutaneous space of groin, H＆E staining showed that they survived and generated 3-dimensional liver-like tissues in which hepatocytes maintained their natural morphology. Conclusion Subcutaneous transplantation of hepatic spheroids can generate hepatic tissue and is thus a novel method for constructing engineered hepatic tissue.