HPLC法同时测定小花鬼针草中3种黄酮类成分含量

HPLC based simultaneous three flavonoid contents determination for Bidens parviflora Willd.

建立了HPLC法同时测定小花鬼针草中芦丁、金丝桃苷和槲皮苷含量的方法.采用Diamonsil C18色谱柱（4.6mm×250 mm,5 μm）,流动相为乙腈-0.2％冰醋酸梯度洗脱,流速为1 mL/min;检测波长为360 nm.结果表明,芦丁、金丝桃苷和槲皮苷在0.50～ 12.50 μg/mL（r =0.999 9）、0.49～ 12.35 μg/mL（r =0.999 8）、0.42～10.55 μg/mL（r =0.999 5）范围内线性良好,平均回收率为99.37％ （RSD =0.56％）、99.35％ （RSD =0.57％）、99.68％ （RSD =0.56％）.该方法简便、准确、重现性好,可用于同时测定鬼针草中3种黄酮成分的含量.

We establish a HPLC based method for determining the contents of rutin,hyperoside and quercetin from Bidens parviflora Willd.The linear ranges of rutin,hyperoside and quercetin are 0.50 ～ 12.50 μg/mL（r=0.9999）,0.49 ～ 12.35 μg/mL（r =0.999 8）,0.42 ～ 10.55 μg/mL （r =0.999 5）,and the average recovery rates （n =3） are 99.37％ （RSD =0.56％）,99.35％ （RSD =0.57％）,99.68％（RSD =0.56） under the conditions of Diamonsil-C18 column,mobile phase of acetonitrile-0.2％ glacial acetic acid,flow rate of 1 mL/min and the detection wavelength of 360 nm.The method is simple,accurate and repeatable and can be employed to determine three flavonoid contents from Bidens parviflora Willd.