摘要
为制备抗3α-HSD蛋白单克隆抗体(MAb),以已表达纯化的高纯度3α-HSD蛋白作为免疫原免疫BALB/c小鼠,利用杂交瘤技术,经过免疫和细胞融合后筛选出特异性的抗3α-HSD蛋白单克隆抗体,成功冻存35株IgG类型阳性杂交瘤细胞株。对14号和20号Anti-3α-HSD进行亲和性测试,发现其与可溶性抗原蛋白的亲和常数分别为3.7E+09和4.2E+09,二者可与抗原牢固结合,具有良好的亲和性。
For development of monoclonal antibody(MAbs) against 3α-HSD, BALB/c mice were immunized with a synthesized 3α- HSD. The splenocytes of the immunized mice were fused with sp2/0 cells,and the positive hybridoma cells were screened. 35 of MAbs were identified as IgG and successfully cryopresevated, the affinity constant of 14# and 20# MAbs against 3α-HSD were 3.7E+09 and 4.2E+09.The affinity of MAbs was good.
出处
《天津农业科学》
CAS
2014年第5期31-35,共5页
Tianjin Agricultural Sciences
基金
质检公益性行业科研专项(201110018-04)
质检总局专项(2013IK173
2013IK293)
关键词
3α-HSD
单克隆抗体
制备
鉴定
3α-HSD
monoclonal antibody (MAbs)
development
identification