纳米银抗菌钛表面对成骨细胞早期生物学行为的影响

Effect of silver nanoparticles modified titanium surface on biological performance of osteoblasts

目的探讨纳米银抗菌钛板对成骨细胞早期生物学行为的影响。方法采用水相硅烷化的方法制备纳米银抗菌钛板(实验组),并以钛表面抛光处理(光滑组)及湿化学处理(湿化学处理组)作为对照,将成骨细胞接种于三组样品表面,检测样品表面细胞形态、细胞增殖及碱性磷酸酶(ALP)活性,分析3组样品成骨细胞早期生物学行为的变化。结果实验组细胞形态明显优于光滑钛片,成骨细胞在3组样品表面细胞增殖及碱性磷酸酶活性检测差异无统计学意义(P>0.05)。结论经过纳米银改性后的钛片对成骨细胞早期生物学行为无不良影响。

Objective The aim of this study was to develop a novel antibacterial titanium implant surface and to test its biolog- ical performance. Methods A silver nanoparticle-modified titanium （Ti-nAg） surface was prepared using silanization method. In the present study,we assessed the differentiation and proliferation of osteoblasts on our Ti-nAg plate by using a scanning electron microsco- py, MTT assay and alkaline phosphatase （ALP）activity. At the same time, polishing treated titanium surface and wet chemical processed titanium surface were used as controls. Results The form of osteoblasts on the Ti-nAg surface was much better than that on the pol- ished Ti. However,there was no significant difference in cell differentiation and ALP activity among the three groups. Conclusion The results demonstrate that the Ti-nAg do not show any cytotoxicity to osteoblasts.