血管紧张素转换酶基因多态性与肺癌的相关性分析

Association of genetic polymorphism of angiotensin converting enzyme gene with lung cancer

目的探讨我国北方地区汉族人群血管紧张素转换酶(ACE)插入、缺失基因多态性与肺癌的相关性。方法选择首都医科大学附属北京胸科医院收治的肺癌确诊患者120例为病例组,按照病理结果分为小细胞癌(SCLC)组31例和非小细胞癌(NSCLC)组(鳞癌、腺癌、鳞腺癌)89例。对照组为健康体检者62例。采用聚合酶链反应-序列特异性引物法(PCR-SSP)进行ACE插入(I)、缺失(D)基因分型分析及多态性分布情况。结果病例组D/D基因型频率明显高于对照组,差异有统计学意义(χ2=4.347,P=0.037,OR=2.85)。小细胞肺癌组(SCLC)的D/D基因型频率明显高于非小细胞肺癌组(NSCLC),差异有统计学意义(χ2=4.602,P=0.032,OR=2.900);SCLC组D等位基因频率明显高于NSCLC组(χ2=5.185,P=0.023,OR=1.967);SCLC组I等位基因频率明显低于NSCLC组(χ2=5.185,P=0.023,OR=0.508)。ACE插入、缺失基因多态性在肺癌各期和NSCLC患者中的频率分布差异无统计学意义(P>0.05)。结论中国北方地区汉族人群ACE基因多态性与肺癌具有相关性,D/D基因型和D等位基因可能是小细胞肺癌的易感因素。

Objective To analyze the association of insertion （I） and deletion （D） genetic polymorphism of angiotensin converting enzyme gene （ACE） with lung cancer in HaM nationality of North China. Methods 120 patients with lung cancer diagnosis in Beijing Chest Hospital, Capital Medical University were selected as case group, they were all divided into SCLC group with 31 patients and NSCLC group （squamous carcinoma, adenocarcinoma, squamous adenocarcinoma） with 89 patients. 62 healthy individuals were chosen as control group. The polymorphisms of ACE-I/D were detected with polymerase chain reaction-sequence special primer （PCR-SSP）; the polymorphism distribution was analyzed. Results The frequency of D/D genetype in case group was higher than that in control group, the difference was statistically significant （χ^2= 4.347, P = 0.037, OR = 2.85）. The frequency of D/D genetype in SCLC group was higher than that in NSCLC group, the difference was statistically significant （χ^2 = 4.602, P = 0.032, OR = 2.900）. The frequency of D allele in SCLC group was higher than that in NSCLC group （χ^2 = 5.185, P = 0.023, OR = 1.967）. The frequency of I allele in SCLC group was lower than that in NSCLC group （χ^2= 5.185, P = 0.023, OR = 0.508）. The difference of distribution of ACE genotypes was not statistically significant in different stages of lung cancer and types of NSCLC patients （P 〉 0.05）. Conclusion This preliminary study shows that ACE genotypes polymorphisms are associated with lung cancer in HaM nationality of North China. The D/D genotype and D allele of ACE may be the susceptible factor of SCLC.