高效液相色谱-蒸发光散射检测法测定消渴丸中黄芪甲苷的含量

Determination of astragaloside in Xiaoke Pills by HPLC-ELSD

目的建立消渴丸中黄芪甲苷的含量测定方法。方法用高效液相色谱法，以蒸发光散射检测器测定黄芪甲苷的含量。色谱柱为Thermo—C18（4．6mm×250mm，5um），流动相为乙腈-水（32：68），流速为：1．0mL·min-1；ELSD参数气体流速为1．60mL·min-1，漂移管温度为80℃；进样量为20uL。结果黄芪甲苷进样量在1．016～4．064ug范围内呈现良好的线性关系，r：0．9998（n=5）；平均回收率为97．03％，RSD=1．8％（n=9）。结论所建立的含量测定方法操作简便、快速、准确、重现性好，可作为消渴丸的质量控制。

Objective To establish a method for the determination of astragaloside in Xiaoke Pills. Methods The HPLC - ELSD was conducted on a Thermo - ClS column （4.6 mm × 250 mm,5 um ） with acetonitrile - water （ 32：68 ） as mobile phase, the flow rate was 1.0 mL · min - 1. The gas flow rate of ELSD parameter was 1.60 mL · min - 1. The drift tube temperature was 80℃ ,the injection volume was 20 uL. Results The linear range of astragaloside was 1. 016 - 4. 064 ug, r = 0. 999 8 （ n = 5 ） , the recovery rate was 97.03 % , RSD = 1.8 % （ n = 9 ）. Conclusion The established HPLC - ELSD was simple ,rapid ,accurate ,reproducible ,and can be used for the quality control of Xiaoke Pills.