宫颈癌中激活蛋白1通路的活化及临床意义

Clinical significance of AP-1 pathway alterations in cervical carcinoma

目的探讨激活蛋白1(AP-1)信号通路的重要组分c-jun和c-fos在宫颈癌中的表达及临床意义。方法免疫组织化学(SP)法检测c-jun和c-fos在70例宫颈鳞状细胞癌、30例宫颈上皮内瘤变及20例慢性宫颈炎中的表达情况,同时分析其与宫颈癌临床病理特征和预后的关系。结果 70例宫颈癌中c-jun和c-fos的阳性表达率分别为57.1%(40/70)、60.0%(42/70),在30例宫颈上皮内瘤变的表达阳性率分别为53.3%(16/30)、63.3%(19/30),而在20例慢性宫颈炎的表达阳性率均为0;宫颈癌组与宫颈炎组相比差异有统计学意义(P<0.01),宫颈癌组与宫颈上皮瘤病变组相比差异无统计学意义(P>0.05)。c-jun和c-fos表达与宫颈癌的临床分期、组织学分级、淋巴结转移等分组中差异具有统计学意义(P<0.05),但与患者年龄及肿瘤的大小等分组中的表达差异无统计学意义(P>0.05)。c-jun和c-fos在宫颈癌的表达呈正相关(r=0.67,P<0.05)。结论 AP-1信号通路活化可能与宫颈鳞状细胞癌发生发展相关,c-jun和c-fos在致瘤过程中可能起协同作用。

Objective To investigate the expression and clinical significance of activator protein-1 （AP-1）,an important sig-naling pathway component of c-jun and c-fos,in cervical cancer .Methods Immunohistochemical （ SP） method was used to detect the expression of c-jun and c-fos in 70 cases of cervical squamous cell carcinoma ,30 cases of cervical intraepithelial lesions and 20 cases of chronic cervicitis .The relationship between expressions and clinicopathologic characteristics and prognostic significance was analyzed . Results The positive expression rates of c-jun and c-fos were 57.1%（40/70） and 60%（42/70） in 70 cases of cervical squamous cell carcinoma.The positive expression rates were 53.3%（16/30） and 63.3%（19/30） in 30 cases of cervical intraepithelial neopla-sia.The positive expression rate in the 20 cases of chronic cervicitis was 0.The expression rate in cervical cancer group and the chronic cervicitis group has a significant difference （P＆lt;0.01）,in cervical cancer and cervical intraepithelial neoplasia group has not statisti -cally significant difference （P＆gt;0.05）.The different expression of c-jun and c-fos protein in cervical carcinomas was statistically signif-icant in the different groups of clinical stage ,pathology grading and lymph node metastasis （P＆lt;0.05）,but was not statistically signifi-cant in the different groups of tumor size and the patient age （P＆gt;0.05）.Additionally,the expression of c-jun was positively correlated with that of c-fos （r=0.67,P＆lt;0.05）.Conclusion AP-1 pathway alterations may play a critical role in the development of cervical cancer,and c-jun and c-fos make synergistic action during the process of cervical cancer .