培元抗感口服液急性毒性及抗炎实验研究

Experimental research for acute toxicity test and anti- inflammatory of Peiyuan Kanggan oral liquid

目的观察培元抗感口服液对昆明种小鼠的急性毒性反应、半数致死量(LD50)及抗炎作用,为临床安全用药提供参考依据。方法培元抗感口服液(培元抗感口服液原液,0.41 g/mL)20.5 g/kg,给药容积0.5 mL 10 g体质量,每日给药2次,间隔2 h。连续14 d,观察用药后小鼠的反应及死亡率。采用二甲苯致小鼠耳廓肿胀,以阿司匹林为阳性对照药,观察培元抗感口服液对急性炎症的影响。结果培元抗感口服液的小鼠最大耐受浓度(MTD)﹥20.5 g/kg(相当推荐临床用量的100倍)。培元抗感口服液8.2 g/kg组小鼠耳廓肿胀明显低于空白对照组(P<0.05)。结论培元抗感口服液的MTD﹥20.5 g/kg;对二甲苯所致的急性炎症有一定的抑制作用。

Objective To observe the acute toxicity reaction and LD 50 of Peiyuan Kanggan oral liquid for single dose on Kunming mice and to provide a reference for clinical drug safety .Methods Mice received Peiyuan Kanggan oral liquid （stoste of Peiyuan Kanggan oral liquid 0.41 g/mL） 20.5 g/kg, 0.5 mL/g （body mass）, twice a day.Response and mortality were observed in mice after treatment for continuous 14 days.Anti -inflammatory effects of Peiyuan Kanggan oral liquid were observed by xylene -induced auricle swelling method and Aspirin as a positive control.Results The maximum tolerated dose of Peiyuan Kanggan oral liquid on mice is greater than 20.5 g/kg （equal to recommended clinical dose 100 times）.Mice auricle swelling in Peiyuan Kanggan oral liquid 8.2 g/kg group was lower than that in blank control group （ P 〈0.05）.Conclusion The maximum tolerated dose of Peiyuan Kanggan oral liquid is greater than 20.5 g/kg and Peiyuan Kanggan oral liquid has some anti -inflammatory effects on xylene-induced acute inflammation .