白藜芦醇对坐骨神经结扎小鼠空间记忆能力的影响

Effects of resveratrol on spatial memory ability in chronic constriction injury of sciatic nerve mice

目的 观察腹腔注射白藜芦醇对坐骨神经结扎小鼠空间记忆能力的影响.方法 SPF级C57BL/6小鼠44只按随机数字表法分为4组：假手术组、坐骨神经结扎模型（CCI组）、白藜芦醇前给药组、白藜芦醇后给药组.CCI组、白藜芦醇前给药组和后给药组制备坐骨神经慢性挤压伤（CCI）模型,假手术组仅暴露坐骨神经不结扎.白藜芦醇前给药组在模型制备前30 min腹腔注射白藜芦醇100 mg/kg,白藜芦醇后给药组在模型制备后14 d腹腔注射白藜芦醇100 mg/kg.模型制备后用全自动小鼠智能鼠笼来持续监测小鼠的空间记忆能力.结果 （1）白藜芦醇前给药组在术后第17～21天与CCI组相比[第17天：（55.80±7.66）％,（51.20±7.94）％;第18天（60.20±3.89）％,（49.80±8.61）％;第19天（62.20±7.25）％,（51.20±6.83）％;第20天（63.00±9.69）％,（48.40±8.84）％;第21天（56.80±7.52）％,（47.20±4.54）％]（P＜0.05）出现了空间记忆能力的提高.（2） CCI组在术后第26～ 28天与假手术组相比[第26天（37.50±5.50）％,（51.80±9.01）％;第27天（37.25±4.19）％,（51.20±5.76）％;第28天（42.25±3.50）％,（52.80±7.52）％]（P＜0.05）出现了空间记忆能力的下降,而白藜芦醇后给药[第26天（46.60±5.27）％,第27天（54.00±7.31）％,第28天（52.60±4.39）％]可以改善空间记忆能力的损伤（与CCI组相比,P＜0.05）.结论 白藜芦醇可以改善坐骨神经结扎的小鼠空间记忆能力的损伤.

Objective To investigate spatial memory ability of intraperitoneal injection of resveratrol in a mice model of chronic constriction injury of sciatic nerve（CCI）.Methods Forty-four C57BL/6 mice were divided randomly into 4 groups：sham group （n=14）,CCI group （n=14）,resveratrol pre-treatment group （i.p.resveratrol 100 mg/kg 30 minutes before CCI model,n=8） and resveratrol post-treatment group （i.p.resveratrol 100 mg/kg 14 days after CCI model,n =8）.CCI group,resveratrol pre-treatment group and resveratrol post-treatment group were operated with the model of neuropathic pain induced by chronic constriction injury of sciatic nerve.In shamoperated controls,an identical surgical procedure was performed,except that the sciatic nerve was not ligated.This was accomplished by using intellicage for mice by newbehavior to record their spatial memory after surgery.Results （l） Resveratrol pre-treatment group showed improved spatial memory ability compared with sham group and CCI group during day 17-21 （17 d：（55.80±7.66） %,（51.20±7.94） % ; 18 d：（60.20±3.89） %,（49.80±8.61） % ; 19 d：（62.20±7.25） %,（51.20±6.83） % ;20 d：（63.00±9.69） %,（48.40±8.84） % ;21 d：（56.80±7.52） %,（47.20±4.54） %）（P〈0.05）,compared with CCI group.（2）From day 26,the spatial memory damage was observed in mice with CCI （26 d：（37.50±5.50）%,（51.80±9.01）%;27 d：（37.25±4.19）%,（51.20±5.76）%;28 d：（42.25± 3.50） %,（52.80± 7.52） %） （P〈 0.05）,compared with sham group.And this damage could be reversed by resveratrol,which was injected when the chronic pain was stable （26 d （46.60± 5.27） %,27 d （54.00± 7.31） %,28 d （52.60±4.39）%）,compared with CCI group（P〈0.05）.Conclusion Chronic constriction injury of sciatic nerve mice due to spatial memory impairment can be improved by resveratrol.