鸡鲍氏3型志贺菌脂多糖及O抗原多糖的提纯与鉴定

Extraction and Identification of Lipopolysaccharide and O-polysaccharide from Chicken S.boydii Type 3

为获得高纯度的鸡鲍氏3型志贺菌脂多糖(lipopolysaccharide,LPS)及其亚单位O抗原多糖(O-polysaccharide,OPS),采用改良热酚水法提取鸡鲍氏3型志贺菌的LPS,并联合使用葡聚糖凝胶层析得到高纯度的LPS;采用酸水解法联合葡聚糖凝胶层析获得高纯度的O-PS。生化方法检测结果显示所提纯LPS和O-PS纯度高,兔回肠襻试验结果显示所提纯的LPS活性好。本试验结果表明提取的LPS和O-PS可进行特异性研究及制备有效的候选疫苗。

To obtain LPS and its subunit O- PS with high purity from chicken S. boydii type 3, LPS from chicken Shigella was extracted with modified hot phenol-water method, high purified LPS was obtained through sepbadex gel chromatography, O-PS was obtained by combination with acid hydrolysis method and sephadex gel chromatography. Biochemical assay showed that the purified LPS and O-PS with high purity, rabbit ideal loop experiment demanstrated that there was high activity in puri fled LPS. The result indicated that extracted LPS and O-PS could be used in specific pathogenesis research and developed as ef fective vaccine candidates.