梅花鹿颈背部异常松弛皮肤光镜组织学观察

Histological Observation of Abnormal Laxity on the Nape of the Neck in Sika Deer by Light Microscope

本研究利用Weigert’s-Van Giesson染色法和Masson氏三色染色法分别对正常梅花鹿和颈背部皮肤异常松弛的梅花鹿皮肤中的弹性纤维和胶原纤维进行观察研究,探讨梅花鹿松弛皮肤组织学致病特点。结果表明,正常梅花鹿真皮网状层中有较多的弹性纤维有规律地平行排列,呈波浪状,染色均匀;真皮乳头层有少量弹性纤维垂直于表皮分布。皮肤松弛梅花鹿或是真皮乳头层中缺乏弹性纤维(大0号和79号鹿),或是网状真皮层中弹性纤维严重发育不全,出现集聚、断裂现象(33号和79号),且排列紊乱、呈不规则状(大0号);正常梅花鹿皮肤胶原纤维在真皮层中呈多层规律性分布。皮肤松弛梅花鹿胶原纤维束比正常胶原束粗大、胶原纤维束减少、堆积、排列杂乱无序,胶原纤维间表现为严重的稀疏。这些现象与人类皮肤松弛症的皮肤组织学症状非常相似。

In this study, elastic fibers and collagen fibers of normal sika deer and abnormal laxity on the nape of neck were observed by Weigert＇ s-Van Giesson staining and Masson staining for investigating the pathogenesis of curls laxa in sika deer. The results showed that there were many elastic fibers in the control group arranged regularly dense, wavy, stained uniform in the reticular dermis ; However, there were few elastic fibers perpendicular to the epidermis in the papillary dermis. Cuis laxa si- ka deer showed inelastic fibers in the papillary dermis （No. 0 and 79） or severe hypoplasia in the reticular dermis, appeared ac- cumulation, breakage （No. 33 and 79）, disorganized, irregular shape （No. 0）. Collagen fibers of the normal deer were distribu- ted multi-layered regularly in the dermis. Collagen bundles of collagen fibers in cutis laxa was markedly thick, reduced, ar- ranged disorderly and severe sparse compared to the normal collagen fibers. It was exactly similar to the histology of cutis laxa skin in human.