凋亡相关基因PDCD5启动子区甲基化在新疆汉族和维吾尔族食管鳞癌中的作用研究

The research of apoptosis-related gene PDCD5 Promoter methylation in esophageal squamous cell carcinoma of Han and Uyghur in Xinjiang

目的探讨凋亡相关基因程序化细胞死亡分子5(PDCD5)启动子区CpG岛在新疆维吾尔族和汉族食管鳞状细胞癌(鳞癌)中的甲基化情况及其与临床病理特征的相关性。方法采用甲基特异性PCR(MSP)法检测40例食管鳞癌患者(维吾尔族20例,汉族20例)癌组织、癌旁组织中PDCD5基因甲基化情况。结果癌组织中PDCD5甲基化阳性率为80.0%(32/40),癌旁组织中甲基化阳性率为35.0%(14/40),差异有统计学意义(P<0.05);PDCD5甲基化阳性率与食管鳞癌临床分期相关(P<0.05);不同民族、性别、年龄食管鳞癌中PDCD5甲基化阳性率差异无统计学意义(P>0.05)。结论在食管鳞癌癌组织中甲基化水平高于癌旁组织;PDCD5甲基化率与食管癌的临床分期有相关性,PDCD5在食管鳞癌中的甲基化率与民族、性别、年龄无相关性。

Objective To investigate the apoptosis-related genes PDCD5 promoter CpG islands methylation in esophageal squamous cell carcinoma tissue of Uygur and Han in Xinjiang.To analyze the ethnic differ-ences,the relationship between them and the clinicopathological characteristics.Methods MSP was used to detect the methylation of PDCD5 in 40 cases of esophageal squamous cell cancer tissues and their adja-cent tissues （20 cases of Uygur,20 cases of Han）.Results The methylation rates of PDCD5 were 80.0%（32/40）in cancer tissues,which is significantly higher than that in adjacent tissues （P〈0.05）.The meth-ylation of PDCD5 is related with clinical staging of esophageal squamous cell carcinoma （P 〈0.05）.There is no significant difference in ethnic group,gender and age （P 〉0.05）.Conclusion The methylation level of PDCD5 in esophageal squamous cell carcinoma tissue is higher than that in adj acent tissues.The methyl-ation of PDCD5 is related with clinical staging of esophageal squamous cell carcinoma.There is no signifi-cant difference in ethnic group,gender and age.