舒芬太尼对2型糖尿病大鼠胸主动脉收缩的影响及机制

目的观察舒芬太尼对去甲肾上腺素(NE)诱发的2型糖尿病(T2DM)大鼠离体胸主动脉血管环收缩功能的影响及可能机制。方法取8只雄性T2DM大鼠,每只大鼠取4段胸主动脉血管环。根据随机原则分为:生理盐水对照组(NC组)、低浓度舒芬太尼组(DS1组,7×10-11mol·L-1)、中浓度舒芬太尼组(DS2组,2×10-10mol·L-1)、高浓度舒芬太尼组(DS3组,1×10-9mol·L-1)。分别悬挂于血管张力测量装置,用60 mmol·L-1的KCl溶液100μL加入浴槽使血管环收缩,记录张力。用K-H液将KCl洗脱后再用生理盐水以及低、中、高浓度的舒芬太尼100μL分别预孵血管环15 min,之后依次向浴槽中加入终浓度为10-8、10-7、10-6、10-5mol·L-1的NE收缩血管,记录每一个NE终浓度下的张力。计算血管环收缩幅度,测量一氧化氮(NO)、内皮素1(ET-1)含量。结果DS1组、DS2组、DS3组与NC组相比血管环收缩幅度降低,NO增多、ET-1减少(P<0.05或0.01)。DS2组、DS3组与DS1组相比血管环收缩幅度降低,NO增多、ET-1减少(P<0.05或0.01)。DS3组与DS2组相比血管环收缩幅度降低,NO增多、ET-1减少(P<0.05或0.01)。结论舒芬太尼可能通过增加血管内皮NO,减少ET-1的分泌,抑制2型糖尿病大鼠离体胸主动脉收缩,且与浓度有关。

Objective To investigate the effect of sufentanil on norepinephrine（NE）-induced contraction of thoracic aorta isolated from rats with type 2 diabetes mellitus（T2 DM）and its mechanism. Methods Eight male rats with type T2 DM were used in the study. The rats were decapitated and their aorta rings were suspended for isometric tension recording. The aortic rings obtained from T2 DM rats were divided into 4 groups（n=8, each）：control group and 3 sufentanil groups （7＆#215;10-11 and 2＆#215;10-10 and 1＆#215;10-9 mol？L-1） The contraction of aortic rings in response to NE and their NO content,ET-1 content in the absence （control） and 3 concentrations of sufentanil 7＆#215;10-11, 2＆#215;10-10 and 1＆#215;10-9 mol？L-1 was recorded. Results The amplitude of NE-induced contraction of thoracic aorta was significantly greater,the NO content was higher and ET-1 content was lower in 3 sufentanil groups than in control group, sufentanil significantly inhibited the NE-induced aortic contraction, increased NO content and reduced ET-1 content in proportion to concentration. Conclusion Sufentanil can inhibit NE-induced contraction of thoracic aorta isolated from rats with T2 DM by increasing NO content, reducing ET-1 content in a concentration-dependent manner.