乙型肝炎表面抗原不同检测方法的优势比较分析

目的：比较化学发光微粒子免疫分析法（CMIA）和酶联免疫吸附法（ELISA）用于乙型肝炎（乙肝）表面抗原检测的优势效果。方法选取2011年3月～2013年4月采用ELISA法与CMIA法对386例患者进行了乙肝表面抗原检测的对比研究。结果 CMIA法检测乙肝表面抗原的阳性率为51.3%，ELISA法阳性率为43.5%，CMIA法阳性率明显更高，与ELISA法比较差异有统计学意义（P〈0.05）；CMIA法的灵敏度与特异性均明显高于ELISA法（P〈0.05）。结论与ELISA法比较，CMIA法对乙肝表面抗原检测在阳性率、灵敏度以及特异性等方面均存在有比较明显的优势，故建议将CMIA法作为临床检测乙肝表面抗原的首选方法而推广应用。

Objective To comparison of chemiluminescence immunoassay particles （Chemiluminescent Microparticle Immuno Assay,CMIA） and enzyme-linked immunosorbent （Enzyme-Linked ImmunoSorbent Assay,ELISA） for the advantage of hepatitis b （HBV） surface antigen detection results. Methods In the past two years by ELISA and CMIA method 386 cases patients were hepatitis B surface antigen were compared. Results CMIA assay for hepatitis B surface antigen positive rate 51.3%, ELISA method positive rate 43.5%, CMIA method was signiifcantly higher compared with the ELISA method was statistically signiifcant （P〈0.05）;CMIA The sensitivity and speciifcity were signiifcantly higher than that of the ELISA method （P〈0.05）. Conclusion Compared with ELISA method, CMIA method for detection of hepatitis b surface antigen in positive rate, sensitivity and speciifcity of existence has obvious advantages, therefore, proposed to CMIA method as the preferred method of clinical detection of hepatitis b surface antigen and popularization and application.