辛伐他汀对大鼠创伤性静脉血栓形成的影响

目的观察辛伐他汀对大鼠创伤性深静脉血栓形成情况以及血栓形成后炎症标志物的变化情况,以探索辛伐他汀的预防血栓形成及抗炎机制。方法取SD大鼠24只随机分为干预组和对照组,每组12只。干预组造模前连续1周喂服普通饲料,同时喂服辛伐他汀,对照组仅喂服普通饲料。2组均造亚急性大鼠创伤性静脉血栓形成模型。造模成功后干预组继续喂服普通饲料同时喂服辛伐他汀,观察组则继续喂服普通饲料。1周后分别采取各组大鼠静脉血行血常规、C反应蛋白(C reactive protein,CRP)及白介素-6(interleukin-6,IL-6)检测,然后处死大鼠,观察大鼠血栓形成情况,比较2组血栓形成的相对危险度(RR),同时比较炎症反应情况。结果对照组比干预组血栓形成的相对危险度(RR)为2.25倍,干预组白细胞、CRP及IL-6显著低于对照组(P<0.05)。结论辛伐他汀能减轻大鼠下肢静脉血栓的发生率,对大鼠下肢静脉血栓有治疗作用,能明显减轻炎症反应,改善大鼠下肢静脉血栓形成的预后。

Objective To observe the effect about vein thrombosis and inflammation changes on rats’femoral vein which treated by simvastatin undergoing trauma. So we can explore the mechanism both preventing vein thrombosis and anti-inflammatory about simvastatin. Methods We divided randomly 24 SD rats into intervention group and control group, with 12 s in each group. The intervention group was fed both normal diet and simvastatin for 1 week before molding, while the control group with common feed only. The two groups were made with subacute venous thrombosis model. After modeled, the intervention group was kept to take normal diet and simvastatin, and the observation group with common diet. Collected venous blood all of them 1 week later.We counted white blood cell with whole blood each of them, and detected C-reactive protein（CRP）, interleukin-6（LI-6） with serum, and then sacrificed all the rats to observe thrombosis in femoral vein,so that we can compare the relative risk （RR） of the thrombosis incidence the two groups and the inflammatory makers. Results The RR is 2.25 between control group and intervention group in the incidence of Femoral vein thrombosis;white blood cells count, CRP and IL-6 aresignificantly lower respectively in the intervention group（P〈0.05）. Conclusion Simvastatin can reduce the incidence of venous thrombosis in rats.It can significantly reduce the inflammatory reaction, improve the prognosis of venous thrombosis in rats.