摘要
为建立制备大量高纯度汉黄芩素的方法,将中药黄芩50g用pH 5水水解12h,再用95％的乙醇提取,得到的提取物浸膏,过30～60目的聚酰胺树脂柱纯化,将含有汉黄芩素的洗脱物采用高速逆流色谱（HSCCC）进行分离.通过该方法制得汉黄芩素525mg,纯度为98.7％.
To develop a method for large scale preparation of high-purity Wogonin from Radix Scutellariae. 50g of Radix Scutellariae was hydrolyzed in water （pH 5） for 12h, and then extracted with 95% ethanol. The extractum was seperated with 30 - 60 mesh polyamide resin column. Fraction with Wogonin was subjected to high-speed countercurrent chromatography （HSCCC） . 525mg Wogonin is obtained with a purity 98.7 %. Thus an effective method for preparing high-purity Wogonin was established.
出处
《亚太传统医药》
2014年第9期33-35,共3页
Asia-Pacific Traditional Medicine
关键词
汉黄芩素
黄芩
提取
聚酰胺柱
纯化
高速逆流色谱
Wogonin
Radix Scutellariac
Extraction
Polyamide Resin Column
Purifion
HSCCC
