肿瘤坏死因子相关凋亡诱导配体诱导的细胞凋亡在再生障碍性贫血发病中的意义

The significance of TRAIL-induced apoptosis in aplastic anemia

目的通过检测肿瘤坏死因子相关凋亡诱导配体(TRAIL)诱导正常骨髓单个核细胞(BMMNC)凋亡率,及抑制TRAIL凋亡途径后再生障碍性贫血(AA)患者骨髓单个核细胞凋亡率,探讨TRAIL在AA患者骨髓造血细胞凋亡中的作用。方法采用流式细胞术检测43例骨髓象正常者(正常组)及TRAIL作用后(TRAIL组)其骨髓单个核细胞凋亡率;14例AA患者(AA组)及TRAIL抗体作用后(TRAIL-Ab组)其骨髓单个核细胞凋亡率。结果 (1)AA组凋亡率较正常组明显增高,差异有统计学意义(P<0.05)。(2)TRAIL组(浓度为30 ng/ml,作用24 h)凋亡率[(39.98±4.594)%]高于正常组[(19.90±6.656)%],差异有统计学意义(P<0.05)。(3)TRAIL-Ab组凋亡率[(30.28±4.594)%]较AA组[(51.29±7.355)%]减少,差异有统计学意义(P<0.05)。结论一定浓度的TRAIL可使正常骨髓单个核细胞凋亡增加,抑制TRAIL诱导的凋亡途径后,AA患者骨髓单个核细胞凋亡减少,提示TRAIL诱导的细胞凋亡,可能在AA发病中发挥一定作用。

Objective To explore the role of tumor necrosis factor related apoptosis inducing ligand （TRAIL） on aplastic anemia bone marrow mononuclear cell（BMMNC）, and approach its effect on the apoptosis of haemopoietic stem cell. By detecting TRAIL induction of normal BMMNC apoptosis rate, and the aplastic anemia BMMNC apoptosis rate after blocking the TRAIL apoptotic pathway. Methods We use flow cytometry to detect the BMMNC apoptosis rate including 43 cases （normal group） and after TRAIL effecting （TRAIL group）; 14 cases with aplastic anemia （AA group） and after TRAIL antibody effecting （TRAIL-Ab group）. Results （1）Aplastic anemia apoptosis rate was higher than normal group. （2）The apoptosis rate （39.98±4.594）%of TRAIL group（concentration of 30 ng/ml, 24 h） was higher than the normal group （19.90±6.656）%（P〈0.05）. （3）The apoptosis rate of TRAIL-Ab group （30.28±4.594）%was lower than aplastic anemia group （51.29±7.355）%（P〈0.05）. Conclusions The TRAIL of a certain concentration can make normal BMMNC apoptosis increases, if block the apoptotic pathway induced by TRAIL, the BMMNC apoptosis of aplastic anemia patients will decrease, suggesting that the cell apoptosis induced by TRAIL may play a role in the onset of aplastic anemia.