雷公藤红素对破骨细胞及胶原诱导关节炎小鼠中趋化因子CXCL2表达的影响

The Effect of Celastrol on Chemokine CXCL2 in Osteoclast and CIA Mice

目的探讨雷公藤红素(Celastrol,Cel)通过抑制趋化因子CXCL2的表达减少大鼠骨髓诱导的破骨细胞形成及胶原诱导的关节炎小鼠(CIA小鼠)模型局部关节炎症及骨侵蚀的发生。方法体内实验制备CIA小鼠模型,分为空白对照组、CIA组及Cel组,以病理染色的方法观察小鼠关节炎症,以实时荧光定量PCR及ELISA的方法检测关节局部及血清的CXCL2表达的变化;体外实验诱导大鼠骨髓形成破骨细胞,加入不同浓度的Cel,通过实时荧光定量PCR检测CXCL2的变化。结果破骨前体细胞及CIA小鼠中CXCL2高表达,且Cel能够抑制其表达,且成剂量依赖性。结论 Cel可能通过抑制趋化因子CXCL2的表达,抑制炎性细胞对组织的浸润,从而减少组织破坏。

Objective To investigate the effect of celastrol on reducing osteoclast formation in rat bone marrow culture and occurrence of local inflammation and bone erosion in Collagen-induced arthritis（CIA）mice by inhibiting the expression of chemokine CXCL2. Methods CIA models of mice were randomly divided into three groups：control group,arthritis group and celastrol group, observing the inflammation by pathological staining technique,detecting the changes of CXCL2 expression in local joints and blood serum by real-time fluorescence quantitative PCR and ELISA. Osteoclast was induced by bone marrow in rat in which celastrol of different concentration was added in vivo test,detecting the changes of CXCL2 expression by real-time fluorescence quantitative PCR. Results High CXCL2 expression in pre-osteoclast and CIA mice was observed. And celastrol can inhabit its expression in dose-dependent manner. Conclusion cellmay inhibit the expression of chemotactic factor CXCL2 and inflammatory cells from invading the organization,thus reducing the tissue destruction.