摘要
目的探讨黄芪注射液对SD大鼠心肌缺血再灌注损伤(MIRI)中Smad3、Smad7表达的影响和机制。方法采用结扎左冠状动脉的方法制备心肌缺血再灌注损伤动物模型。32只健康成年SD大鼠,体质量(230±20)g,平均分为假手术组、缺血再灌注组、辛伐他汀组和黄芪预处理组。通过免疫组织化学和RT-PCR,分别测定Smad3蛋白、Smad7蛋白及Smad3mRNA、Smad7mRNA的表达。计算各组中心肌细胞的凋亡率。结果与假手术组相比,缺血再灌注组心肌细胞的凋亡率明显增加(P=0.000),并且Smad3蛋白和Smad3mRNA表达增加(P=0.000),Smad7蛋白和Smad7mRNA表达减少(P=0.000);与缺血再灌注组相比,黄芪注射液组(P=0.000)和辛伐他汀组(P=0.000)可明显降低心肌细胞的凋亡率;Smad3蛋白和Smad3mRNA表达减少(P=0.000);而Smad7蛋白和Smad7mRNA表达增加(P=0.000)。结论黄芪注射液可以上调SD大鼠缺血再灌注损伤时心肌细胞Smad7蛋白和Smad7mRNA表达,下调Smad3蛋白和Smad3mRNA表达。
Objective To explore the effect and mechanism of astragalus injection on Smad3 and Smad7 expression caused by myocardial ischemia reperfusion inj ury（MIRI）in SD Rats.Methods The MIRI model was established by ligating the left anterior descending coronary artery.Thirty two SD rats were randomly and equally divided into four groups named by sham,model,simvas-tatin and astragalus pretreatment after one week′s adaptive feeding.The protein and mRNA expressions of Smad3 and Smad7 were respectively detected by immunohistochemical staining and RT-PCR in cardiomyocyte,in order to analysis the relationship between Smad3′s and Smad7′s protein and mRNA expressions and calculate each group′s myocardial cell apoptosis rate.Results Compared with the Sham group,ischemia reperfusion myocardial cells apoptosis rate increased significantly（P=0.000）,the protein and mR-NA expression of Smad3 increased（P=0.000）but the Smad7 decreased（P=0.000）.Compared with the ischemia reperfusion,as-tragalus injection group and simvastatin group can significantly reduce the apoptosis of myocardial cells（P=0.000）,The protein and mRNA expression of Smad3 decreased（P=0.000）but the Smad7 increased（P=0.000）.Conclusion Astragalus injection can affect the apoptosis of myocardial cells in ischemia reperfusion inj ury of the SD rat myocardial cells,which is realized by up-regula-ting Smad7 protein and Smad7mRNA expression,down-regulating Smad3 protein and Smad3mRNA expression.
出处
《重庆医学》
CAS
CSCD
北大核心
2014年第13期1597-1599,共3页
Chongqing medicine
