穴位埋药线对难治性癫痫大鼠大脑海马及皮质多药耐药基因MDR1b的影响

Effects of Drug Line Buried on Acupuncture Point on the Expressions of Multiple Drug Resistant Gene in Intractable Epileptic Rats Induced by Kainic Acid

目的探讨穴位埋药线对难治性癫痫大鼠大脑海马及皮质中多药耐药基因MDR1b的影响。方法选用雄性SD大鼠120只,随机分为空白对照组、模型组、拉莫三嗪组、普通线组、药线组。普通线组与药线组均取大椎、肝俞(双)、血海(双)、丰隆(双)穴,先埋一侧穴位,间隔15 d后埋对侧穴位;拉莫三嗪组按照人用拉莫三嗪剂量为5 mg/(kg·d)换算灌胃大鼠,每日2次;模型组给予灌胃同等体积(2 mL/d)的蒸馏水,均干预30 d。采用荧光实时定量聚合敏链反应(RT-PCR)的方法检测多药耐药基因MDR1b在海马与颞叶皮层的表达。结果与模型组相比,药线、普通线、拉莫三嗪干预后能降低致痫大鼠海马区多药耐药基因MDR1b的表达水平(P<0.05);与空白对照组比较,模型组中致痫鼠海马区多药耐药基因MDR1b的表达水平明显升高(P<0.01);与普通线组比较,药线组明显降低致痫大鼠海马区多药耐药基因MDR1b的表达水平(P<0.05);模型组的海马区与颞叶皮质区多药耐药基因表达差异有统计学意义(P<0.05)。结论埋药线逆转与降低致痫大鼠海马区及皮质区多药耐药基因的表达水平,可能是其抗癫痫生物学作用机制之一。

Objective To investigate the effects of acupuncture therapy on buried intractable epilepsy hippocampus and cerebral cortex of multidrug resistance gene MDR1b. Method Hip- pocampal dosing can be located and was injected kainic acid to induce epilepsy modelby microsyringe according to Paxions Watras map and under the guidance of brain ster-eotaxic apparatus. The rats whose seizure behavior level in Racines standard V level or above were intragastric injection administration with valproate and carbamazepine intervention for 14 days, Sub-convulsions dose was injected from micro-catheter again to kindle the seizure, the rats whose seizure behavior level in racines standard V level or above and electroencephalogram （EEG） test with epileptiform wave discharge were selected to be successful resistant refractory epilepsy rats model. Buried ordinary wire line group and the drug line group were taken Dazhui, Ganshu （double）, Xuehai （double）, Fenglong （double） acupoints. First buried side points, spaced 15 days buried in the contralateral acupoints. According to 0.1 mg/kg.d Lamotrigine concentr-ations. Lamotrigine group rats were fed 2 times a day. Model group were given the same volume gavage （2 mL/d） of distilled water were administered 30 days. To detect MDR1b multi-resistant genes expression by real-time fluorescent quantitative aggregate min chain reaction （RT-PCR） methods. Results Multidrug resistance gene test results of hippocampus and cortex showed that after lamotrigine, drug lines buffed, common line buried intervention could reduce epileptic rat hippocampus MDR1b multidrug resistance gene expression level （P〈0.05）, Compared with the model group; A comparison with the control group, model group was significantly higher expression levels in the hippocampus of epileptic rats of MDR1b （P〈0.01）; Compared with the general line group buried, drug-line buried of expression levels were significantly lower than the hippocampus of epileptic rats of MDR1b （P〈0.05）; Between Hippocampus and temporal cortex of the model group of MDR1b the difference expression area was statistically significant （P〈0.05）. Conclusion Expression of multidrug resistance gene MDR1b presence of chronic brain lit KA rat hippocampus compared with drug-resistant epilepsy model was significantly higher cortical areas; Buried line reversal and reduce drug-induced epileptic rat hippocampus expression levels of multidrug resistance gene may be one of the mechanisms of the biological effects of anti-epileptic.