心复康口服液对心肌梗死后心衰大鼠心肌组织mit-CK mRNA及蛋白表达的影响

Effect of Xinfukang Oral Liquid on the Expression of mit-CK mRNA and Protein in Rats with Heart Failure after Myocardial Infarction

目的观察心复康口服液对心肌梗死心衰大鼠心肌组织线粒体肌酸激酶(mit-CK)mRNA及蛋白表达的影响。方法采用结扎SD大鼠左冠状动脉前降支复制心肌梗死后心衰大鼠模型,用心复康口服液于术后24h灌胃给药至6周;以卡托普利作为阳性对照药,观察大鼠梗死周围心肌组织mit-CK mRNA及mit-CK蛋白表达水平。结果第6周末,与模型组比较,卡托普利组、心复康口服液组心肌mit-CKmRNA、mit-CK蛋白表达均上调(P<0.01)。第6周末,与卡托利组比较,心复康口服液组心肌mitCKmRNA表达水平下调(P<0.05),心肌mit-CK蛋白表达水平差异无统计学意义(P>0.05)。结论心复康口服液可上调心肌梗死后心衰大鼠心肌细胞mit-CKmRNA的表达,促进mit-CK蛋白含量增加,达到改善心肌细胞能量穿梭紊乱,重塑心肌梗死后心衰大鼠心肌能量代谢作用。

Objective To study the effect of Xinfukang oral liquid（XOL） on the expression of mitochondrialcreatine kinase（mit - CK） mRNA and protein in rats with heart failure after myocardial infarction. Methods The rat models of heart failure after myocardial infarction were created by ligating their left anterior descending coronary arteries, after 24 hours of creating models, rats in drug groups were fed on XOL and eaptopril through gastotube for 6 weeks,while eaptopril group was served as control. The expression of mit - CK mRNA and protein around infarction area were observed. Results Compared with mode group, the expression of mit - CK mRNA and protein in captopril group and XOL group were increased at 6th weekend （P〈0.01）. Compared with captopril group, the expression of mit - CK mRNA in XOL group was decreased at 6th weekend （P〈0.05）. While there was no significant difference in mit - CK protein expression between eaptopril group and XOL group at 6th weekend （P〈0.05）. Conclusion XOL can effectively increase the expression level of mit-CK mRNA, as well as the protein content of mit-CK of rats with heart failure after myocardial infarction, and thus help to improve heart failure myocardial energy shuttle and remodel the process of myocardial energy metabolism function.