富血小板血浆支架诱导牙髓再生的体内研究

Platelet-rich plasma scaffolds induce dental pulp-like tissue regeneration in vivo

目的探讨不同浓度的富血小板血浆(PRP)支架在体内诱导牙髓组织再生的能力。方法将小型猪乳牙牙根段进行化学预备,采用二次离心法制备PRP,根据注入根管中的成分不同将研究分为4组:(1)阴性对照组,即全血组;(2)100%PRP组;(3)50%PRP组;(4)空白组,即空的牙根段;每组5个样本,分别植入裸鼠背部皮下,于术后5周处死动物,取出样本进行组织学观察。结果植入5周后,100%PRP组根管内充满了炎性细胞,50%PRP组根管内有少量牙髓样的组织生成。结论合适浓度的PRP作为生物支架在体内再生牙髓样的组织是可行的。

Objective To evaluate the in vivo dental pulp regeneration capacity of various concentration of the platelet-rich plasma （PRP）. Methods The tooth roots of minipigs were prepared by chemical methods. PRP was extracted by two-step centrifugation. The roots were divided into four groups according to the kinds of scaffold injected into the root canals （5 roots each group）： （1）control group, whole blood; （2）100% PRP; （3）50% PRP; （4）blank group： root fragments with empty canal space. The roots were implanted subcutaneously in nude mice. The animals were sacrificed after 5 weeks. The samples were taken for histological examination . Results The newly formed pulp-like tissues were observed in the roots which filled 50% PRP scaffolds in canals . The group of 100% PRP scaffolds were full of inflammatory cells. Conclusions It is feasible for the platelet-rich plasma with proper concentration as scaffolds to induce dental pulp regeneration in vivo.