MTUS1/ATIP1对舌鳞癌细胞增殖及凋亡的影响

The effect of MTUS1/ATIP1 on proliferation and apopsis of oral tongue squmous cell carcinoma

目的探讨过表达MTUS1/ATIP1对舌鳞癌细胞增殖及凋亡的影响。方法检测人舌鳞癌系UM1、SCC-9、SCC-15、Tca8113细胞株中MTUS1的表达水平。应用含ATIP1片段的质粒转染舌鳞癌细胞,48 h后MTT检测舌鳞癌细胞的增殖能力;应用流式细胞仪技术和细胞免疫荧光技术检测细胞周期和细胞凋亡率;Western blot检测舌鳞癌细胞中MTUS1、p53、ERK1/2的表达情况。结果转染MTUS1/ATIP1后细胞的增殖明显受到抑制,其抑制率约为40%(t=0.023,P<0.05);高表达MTUS1/ATIP1可导致舌鳞癌细胞株G1期阻滞(G1期:t=0.032,G2期:t=0.036,S期:t=0.027,P<0.05)并诱导细胞凋亡率的明显升高,差异具有统计学意义(t=0.005,P<0.05)。Western blot检测显示,转染MTUS1/ATIP1后ERK的表达升高,磷酸化的ERK表达下降,p53的表达升高。结论 MTUS1可抑制舌鳞癌细胞的增殖,诱导细胞凋亡。

Objective To investigate the role of MTUS1/ATIP1 in suppression proliferation and induce apoptosis of oral tongue squmous cell carcinoma . Methods Investigate the expression levels of MTUS1 in human tongue cell carcinoma cell lines （UM1, SCC-15, SCC-9, Tca8113）. Tongue squamous cancer cell were transfected with a ATIP1 expression vector for 48 h . The Proliferation analysis was performed with MTT assay. Flow cytometry and immunofluorescence technique were employed to measure cell cycle and apoptosis of tongue cell carcinoma cells. The expression levels of proteins, MTUS1, p53, ERK1/2, in tongue cell carcinoma cells transfected with ATIP1 were detected by Western blotting . Results In vitro functional study showed that over-expression of MTUS1/ATIP1 significantly increased the apoptosis rate （P〈0.05）, led to G1 arrest and decreased the proliferation activity, with an inhibition rate of about 40% （P 〈 0.05） in OTSCC cell lines. The over-expression of MTUS1/ATIP1 induced the reduction of phosphor-ylation of ERK and up-regulation of p53 and ERK . Conclusion MTUS1/ATIP1 is suppose to be associated with inhibit proliferation and induce apoptosis in oral tongue squmous cell carcinoma.