不同发酵条件对毛壳菌产木聚糖酶的影响

Study on the Optimum Fermentation Condition for Production of Xylanase by Chaetomiaceae

通过对毛壳菌产木聚糖酶改变发酵条件,探讨毛壳菌木聚糖酶的生产能力及不同酶解条件对毛壳菌产生的木聚糖酶的影响,以确定其最佳培养基及发酵条件。结果表明,以玉米芯4%+蛋白胨0.5%+酵母粉0.5%为发酵基质对产酶最为有利,当温度为39℃、pH4.6、发酵时间3d时产酶效果最好。

The experiment discusses the ability of Chaetomium xylanase production and the different affects of Enzymolysis conditions of xylanase Chaetomium,determine the most suitable medium and fermentation conditions through changing the Chaetomium fermentation condition of xylanase. The result showed that, with corn cob 4%＋peptone 0.5%＋yeast powder 0.5% as fermentation substrate of enzyme is most advanta-geous,when the temperature is 39℃,pH4.6,fermentation time 3D enzyme producing the best effect.