皮状丝孢酵母的高温驯化及细胞膜脂肪酸组成的测定

High temperature domestication of T. and fatty acid composition of its cytomembrane

通过对产油皮状丝孢酵母T.cutaneum CX1进行高温驯化及平板筛选，获得7株耐高温菌株G1～G7。在42℃条件下耐高温菌株G3菌体干重、含油量及合油率分别为11．13、1．33g／L及11．95％，较原始菌株分别提高32．8％、46．2％及10．0％，发酵优势明显。细胞膜脂肪酸组成的测定结果表明，同原始菌株相比，耐高温菌株G3细胞膜中饱和脂肪酸含量更高。当培养温度升高时，耐高温菌株G3细胞膜中饱和脂肪酸含量会进一步上升。

Seven thermophilic strains G1 - G7 were obtained according to the high temperature domestica- tion and plate screening of T. cutaneum CX1. At 42 ℃, the dry weight of strain, oil accumulation and oil content of the thermophilic strain G3 were 11. 13,1.33 g/L and 11.95% respectively, increasing by 32.8% ,46.2% and 10.0% respectively comparing with original strain, so the advantage of fermentation was obvious. The analysis results of fatty acid composition of cytomembrane showed that the thermophilic strain G3 had higher content of saturated fatty acids in cytomembrane than original strain, meanwhile, the content of saturated fatty acids in cytomembrane of the thermophilic strain G3 increased further with the cultural temperature rising.