摘要
目的了解国产血液制品中人博卡病毒(HBoV)的基因组及血清学抗体的流行状况。方法基于ABI7900HT Fast Real-time PCR System平台,建立检测HBoV DNA的特异性、敏感性Real-time PCR(SYBR)方法,并依之对2009—2012年国内3家血液制品公司生产的326份血液制品[65份白蛋白、197份静脉注射丙种球蛋白(IVIG)、47份凝血因子(FⅧ)、12份纤维蛋白原(Fg)和5份蛋白酶原复合物(PCC)]作HBoV DNA的筛查,并用Nested—PCR法确认;对HBoV DNA确认阳性的样品进行抗.HBoV-IgG和IgM筛查。结果部分国产血液制品中HBoV DNA的阳性率为13.50%(44/326);自蛋白、IVIG、FⅧ和Fg的阳性率分别为3.08%(2/65)、7.11%(14/197)、55.32%(26/47)和16.67%(2/12),PCC中HBoVDNA未检出。阳性样品中,有2份FⅧ制品HBoV载量〉10^4cp/mL。33份HBoV基因组阳性血液制品的抗-HBoV-IgG和IgM筛查。所有样品抗-HBoV—IgM均为阴性,33.33%(11/33)的样品抗-HBoV—IgG阳性,抗体滴度为(254.74~336.88)ng/L。结论国产血液制品中存在低载量的HBoV流行。
Objective To offer informations on the genomic and sero-prcvalence of human Bocavirus (HBoV) in partial Chinese plasma derivatives. Methods Based on the ABI 7 900 HT Fast real-time PCR system platform, we established a specific and sensitive real-time PCR(SYBR) assay to detect HBoV DNA. On the basis of method constructed above,we tested 326 plasma derivatives[ including 65 batches of albumin, 197 batches of intravenous immunoglobulinfrom (IVIG) ,47 batches of anti-hemophilic factor Ⅷ (FⅧ), 12 batches of Fibrinogen (Fg) ,5 batches of Prothrombin Complex Concentrate (PCC) ] collected from 3 Chinese blood product manufacturers between 2009 and 2012 for the genomic prevalence of HBoV in different kind of plasma derivatives in China. Nested PCR were performed to confirm the positive samples tested by real-time PCR. HBoV DNA positive samples were also screened for anti-HBoV-IgG and IgM to obtain the status of HBoV antibodies. Results A total of 44 samples were HBoV genome positive in all the plasma derivatives tested in our study, the positive rate is 13.50%. HBoV DNA was detected in 3.08% (2/65) albumin samples,7.11% (14/197) IVIG samples,55.32% (26/47) anti-hcmophilic factor Ⅷ samples, 16.67% (2/12) fibrinogen samples ,respectively ,no HBoV DNA was detected in the five PCC samples. Among all the positive samples ,2 FⅧsamples were quantified with HBoV viral loads over 104 copies/mL. As a result, 33 genomic positive samples were tested for HBoV antibodies,all were negative for anti-HBoV-IgM, 33.33% (11/33) were positive for anti-HBoV-IgG. Conclusion HBoV are detectable in Chinese plasma derivatives at low levels.
出处
《中国输血杂志》
CAS
CSCD
北大核心
2014年第4期370-374,共5页
Chinese Journal of Blood Transfusion