热辣2号辣椒纯度鉴定及优良自交系遗传多样性分析

Purity Identification of Rela No. 2 Pepper and Genetic Diversity Analysis of Excellent Pepper Inbred Lines

为了提高种子质量和充分利用辣椒种质资源。应用SSR分子标记对热辣2号杂交种纯度进行鉴定并对部分辣椒优良自交系进行遗传多样性分析。以热辣2号母本材料CAYB02和父本材料CAYB01为模板筛选85对辣椒SSR引物。其中12对引物在亲本间能扩增出明显的多态性，多态性比率为14．12％，多态性标记分别位于辣椒1、3、4、6、7、9、11、12号染色体，利用3对位于不同染色体上的SSR标记对热辣2号黄灯笼椒杂交种的纯度进行鉴定，热辣2号种子纯度为98．79％，标记鉴定结果与田间鉴定结果一致。研究结果表明．利用SSR标记鉴定辣椒杂交种纯度是切实可行的。利用12对多态性明显、稳定可靠的SSR引物对部分辣椒自交系进行遗传多样性分析，共扩增出60条条带，多态性位点比率为100％．平均每对引物检测出5个等位基因。30份辣椒自交系间遗传相似系数变幅为0.33～1．00，平均为0。65．表明供试材料间具有丰富的遗传多样性。聚类分析结果表明，在遗传相似系数为0．47时，可以将中国辣椒和一年生辣椒区分开来。在遗传相似系数为0.85时．30份辣椒优良自交系分为11个类群．辣椒种质遗传关系与地理来源和农艺性状具有一定的相关性。

In order to improve the seed quality and utilize adequately the pepper germplasm, the hybrid purity of Rela No.2 and the genetic diversity of partial pepper inbred lines were analyzed by SSR markers. 85 pairs of SSR primers were screened for polymorphic markers based on the DNA templates of female parent CAYB02 and male parent CAYB01. Twelve SSR markers were polymorphic between parent materials. The polymorphic ratio was 14.12%. The polymorphic markers were located on chromosome 1, 3, 4, 6, 7, 9, 11, 12, respectively. The hybrid purity of Rela No.2 was identified with 3 SSR markers located on different chromosomes. The hybrid purity was 98.79%. The result was consistent with field assessment. These results clearly demonstrate that SSR markers should be useful for assessing the purity of pepper hybrid. Twelve pairs of polymorphic, stable and reliable SSR primers were used for genetic diversity analysis of partial pepper inbred lines. Sixty bands were amplified from the 12 SSR markers. The percentage of polymorphic loci was 100% with an average of 5 alleles per locus. The genetic similarity coefficients ranged from 0.33 to 1.00 among these inbred lines with an average of 0.65, indicating that there was a significant genetic diversity in these tested materials. The cluster analysis results showed that Capsicum chinense and Capsicum annuum could be separated at the genetic similarity coefficient of 0.47. Thirty pepper inbred lines could be divided into 11 groups at the genetic similarity coefficient of 0.85. The genetic relationship of pepper germplasm was related to geographic origin and agronomic traits.