李亲缘关系SRAP分析

SRAP Analysis of the Genetic Relationship of Prunus L.

利用相关序列扩增多态性(SRAP)标记对巴山脆李及达州地区优良脆李资源进行亲缘关系分析,为巴山脆李品种的鉴定和其他李资源的保护及利用提供理论依据。从180对引物组合中筛选出12对多态性高、扩增谱带清晰的SRAP引物,对22份李资源进行扩增,共获得103条谱带,其中多态性谱带64条,多态性比率为60.34%。平均每对引物组合扩增出多态性谱带5.42条。利用UPGMA法构建树状聚类图,在相似性系数0.68处可将22份样品分成3组。聚类结果与按果形分类结果一致,与其地理位置和熟期也存在一定的相关性。

Providing a theoretical basis for the identification of ＇Bashan Cuili＇ and protection and utilization of other crisp varieties in Dazhou, the sequence related amplified polymorphism was used to analyse the genetic relationship of the Bashan Cuili and the excellent regional resources in Dazhou. Twelve primer pairs out of 180 pairs of sequence related amplified polymorphism （SRAP） markers which were of high polymorphism and showed clear bands were used to analyze the genetic diversity of 22 samples, which generating 103 bands with 64 bands （60.34%） as polymorphie ones. Each primer combination amplified loci was 5.42. A dendrogram was constructed based on the SRAP data using UPGMA cluster method. The 22 samples of plum were classified into 3 major groups when the similarity coefficient was at 0.68. The clustering results and fruit shape was basically corresponded, and was related with resource distribution and mature phase.