番茄SlSnRK2s基因表达在促进叶片衰老中的作用

The Role of SlSnRK2s Expression in Promoting the Leaf Senescence in Tomato

SnRK2(sucrose non-fermenting 1一related protein kinase2)是脱落酸(ABA)信号转导途径中的正调控因子,广泛参与了植物的生长发育、病虫害防御、ABA和非生物胁迫等多种信号的应答反应。从番茄中分离了3个与SnRK2.2、SnRK2.3和SnRK2.6同源的基因,分别命名为SlSnRK2.2、SlSnRK2.3和SlSnRK2.6,同时构建了SlSlSnRK2.2/2.3/2.6 RNAi载体,并通过根癌农杆菌介导的叶盘法转化番茄,得到转基因番茄植株。初步表型分析结果发现转基因植株呈叶片早衰的现象。通过对乙烯信号相关基因的检测可知,转基因植株中除了ERF2表达量低于野生型,其余3个基因(ACO4、ERF1B、ERF1)的表达量均高于野生型,表明SlSnRK2.2、SlSnRK2.3和SlSnRK2.6与叶片衰老有关,可能参与ABA和乙烯信号互作,此结果对揭示植物衰老机理具有重要意义。

SnRK2（sucrose non-fermenting 1-related protein kinase2） is a positive regulators of Abscisic acid （ABA）signaling, which plays a pivotal role in plant development,stress physiology, ABA and abiotic stresses signal responses. In the study, three homolog of SnRK2.2, SnRK2.3, SnRK2.6 genes, named as SISnRK2.2, SISnRK2.3 and SlSnRK2.6, were isolated from tomato（Solanum lycopersicum, cv Micro Tom）. RNAi vectors of SlSInRK2.2/2.3/2.6 were constructed and some phenotype like leaf senescence in transgenie plants were observed. Expression of ethylene-synthesis -related genes was changed in transgenic plants. The results suggested that SlSnRK2s related to plants senescence, and SlSnRK2.2, SlSnRK2.3 and SlSnRK2.6 maybe involved in the signal interaction of ABA and ethylene.