不同矿化方式下丝素蛋白电纺纤维的仿生矿化

Biomimetic Mineralization of Silk Fibroin Electrospun Nanofibers under Different Mineralization Methods

以丝素蛋白静电纺纳米纤维为模板,采用一种新颖的矿化方式——动态交替浸渍法和通常的静态交替浸渍法,对其进行仿生矿化,研究丝素蛋白静电纺纤维膜在不同方式下的矿化特性。通过扫描电镜、X射线衍射及热重分析等手段对矿化纤维的形貌、结构及沉积量进行表征。结果表明:静态交替浸渍法下形成的磷灰石主要沉积在丝素蛋白纤维表面,生长缓慢;相比之下动态交替浸渍矿化法下形成的磷灰石在纤维表面及纤维膜间隙均有沉积,其成核和生长较快,在相同矿化周期下,可形成由纳米微粒聚结而成的较大颗粒。动态交替矿化纤维中磷灰石沉积量相对较高,两种矿化方式下生成的磷灰石均为缺钙型羟基磷灰石。

In this paper,a novel biomimetic mineralization method of dynamic alternate soaking process has been compared with the static alternate soaking method by using electrospun silk fibroin nanofibers as the template to study mineralization features of silk fibroin electrospun fibrous membrane under different methods. The morphology, structure and deposition content of mineralized electrospun SF nanofibers have been characterized by SEM,XRD and TGA. The results show that the apatite formed in static alternate soaking method mainly deposits on the surface of silk fibroin fibers and grows slowly; by contrast,the apatite formed in dynamic alternate soaking method deposits on the fiber surface and fibrous intermembrane space and grows fast. Under the same mineralization period,it can form large particles due to nanoparticle coalescence. It is found that the structure of the apatite particles is calcium-deficient hydroxyapatite both in dynamic and static processes,and the apatite deposition content of mineralized fibers in dynamic process is higher than that of static process.