诊断铁缺乏蛋白芯片检测性能的研究

Study on detection function of protein microarray for iron deficiency diagnosis

目的对蛋白芯片检测平台在诊断铁缺乏人群时的实际检测性能进行研究。方法用干扰性实验分析蛋白芯片检测血清铁蛋白(SF)与可溶性转铁蛋白受体(sTfR)的抗干扰能力;以同一批次点样后的多次重复性检测实验分析其检测稳定性;以对同一实验结果的重复检测分析其检测时效。结果白蛋白、甘油三油酸酯均不对SF、sTfR的检测结果产生干扰;已建立的蛋白芯片检测系统在一次点样后至少可保存38 d;此检测系统的检测时效可以定为22 d。结论诊断铁缺乏的蛋白芯片具有可用于实际应用的检测性能。

Objective To study the reality detect function of protein microarray which diagnosis the iron deficiency （ID）. Methods Evaluated anti-interference ability with interference experiment, analyzed the detection stability with many times repeated experiment, detected the same experiment result repeatedly to analyze the detection indate. Results There was not interference to serum ferritin and soluble transferrin respector from distraction. The protein microarry can be using in 38 d after print probes. The detection indate should be in 22 d. Conclusion The protein microarry that used as diagnosis ID opposed the reality detection ability.