鱼腥草对铜绿微囊藻的抑制作用

Inhibition effects of Houttuynia cordata Thunb. on Microcystis aeruginosa

目的研究鱼腥草(Houttuynia cordata Thunb.)对水华蓝藻铜绿微囊藻(Microcystis aeruginosa)的抑制作用。方法采用鱼腥草水浸提液以及4种有机溶剂(丙酮、乙酸乙酯、石油醚、乙醇)萃取物(粗提物)分别抑藻,通过藻细胞密度计算出抑制率;同时采用GC-MS分析鉴定抑藻效果最好的萃取物中的化感物质。结果鱼腥草水浸提液及4种粗提物对铜绿微囊藻均有很好的抑制作用;4种粗提物的抑藻效果为:乙酸乙酯粗提物>乙醇粗提物>丙酮粗提物>石油醚粗提物;乙酸乙酯粗提物中的化感物质主要有二丙酮醇、2,2-二甲基己酮、氯己酸及4-苯腈酯。结论鱼腥草具有很强的抑藻水华蓝藻作用,具有开发成生态抑藻剂的潜质。

Objective To research the inhibitory effect of Houttuynia cordata Thunb. on Microcystis aeruginosa, netheds M. aeruginosat were treated respectively by H. cordata leaching solution or H. cordata extracts H. cordata leaching solution extracted by water and the H. cordata extracts extracted by organic solvent （ acetone, ethyl acetate, petroleum ether and ethanol, respectively ）. The inhibition ratios were calculated according to the M. aeruginosa densities, and the allelochemicals of the extract that had the best inhibitiory effect on M. aeruginosa were identified by GC-MS analysis~ Results It was proved that leaching solution of H. cordata and four crude extracts had good inhibitory effect on M. aeruginosat. The inhibitory effects of the four crude extracts were the fraction extracted by ethyl acetate, the fraction extracted by ethanol, the fraction extracted by acetone and the fraction extracted by petroleum ether from strong to weak in turn. Then, the allelochemicals of the fraction extracted by ethyl acetate were indentified, mainly including acetonyldimethylcarbinol, 2,2-dimethyl-3-hexanone, 6-chlorohexanoic and 4-cyanophenyl ester. Conclusion H. cordata has strong inhibitory effect on water- blooming cyanobacteria and the potential to develop into an ecological M. aeruginosa inhibiting agent.