蕃拉芦荟水溶性多糖的分离与鉴定

Structural Determination of Polysaccharides Isolated from Aloe barbadensis Miller

以蕃拉芦荟（Ａｌｏｅ ｂａｒｂａｄｅｎｓｉｓ Ｍｉｌｌｅｒ）为材料，从中分离出 ３种多糖ＰＡＢ１、ＰＡＢ２和ＰＡＢ３。 ＰＡＢ１的总糖含量和糖醛酸含量分别为 ７８．５７％和１４．５９％；ＰＡＢ２的分别为 ８８．７２％和 ６．９０％：ＰＡＢ３的分别为 ５０．２３％和 １０．９％，蛋白质含量为 ２８．８１％。红外光谱、气相色谱、酶水解等测定结果表明，ＰＡＢ１为葡萄聚糖，ＰＡＢ２为葡萄甘露聚精，ＰＡＢ３是一种含有甘露糖的糖蛋白。

Three kinds of polysaccharides, PAB1, PAB2 and PAB3,were extracted from Aloe barbadensis Miller. The purity of PAB1, PAB2 and PAB3 was 78.57 %,88.72 %, 50.23 %, and the content of glucuronic acid was 14.59 %, 6.90 % and 10.9% respectively. PAB3 was a glycoprotein with 28.81 % of protein. The result indicated that there might be no linear chain of a but B among the polysaccharides after enzymolysis. Infrared spectrum analysis showed that all had typical absorption peaks of polysaccharide and acetyl. These showed that the three polysaccharides were partially acetylated B pyrone acid het- eropolysaccharides. Gas chromatography analyses revealed that PAB1 was composed of glucose, PAB2 of glucose and mannose, and PAB3 of mannose.