酶解小球藻蛋白制备多肽的工艺研究

Study on Enzymatic Preparation of Polypeptides from Chlorella Protein

以钦州湾小球藻为原料,通过单因素和正交试验探讨酶解法制备多肽的最佳工艺。实验结果表明:酶解小球藻蛋白制备多肽的最佳工艺条件为:pH 6.5,酶解温度30℃,酶解时间20 min,料液比1∶100 g/mL。此时多肽得率为15.538%±0.2%。

In this study, using single factor and orthogonal experiments, the prepare process optimization of polypeptides from chlorella protein of Qinzhou Bay were investigated by enzyme hydrolysis. The results showed that optimum conditions for prepare polypeptides were as follows：pH is 6.5, the hydrolysis temperature and time is 30℃and 20 min, chlorella was mixed with phosphate buffer at ratio of 1∶100 g/mL. At the condition, yield of polypeptides is 15.538%±0.2%.